Project description:In our current study, SisPerR was revealed as a transcriptional repressor in response to oxidative stress in Saccharolobus islandicus REY15A. Deletion of sisperR abolishes the oxidative stress-induced expression of a large number of genes. To identify the target genes directly bound by SisPerR in vivo, chromatin immunoprecipitation-sequencing (ChIP-seq) was performed.
Project description:Sensing of ROS is essential for cellular response to oxidative stress. While oxidative stress response in bacteria and eukaryotes has been extensively studied, less is known about the mechanism in archaea. In this study, we demonstrate that deletion of sisperR, a gene encoding the PerR of the model hyperthermophilic archaeon Saccharolobus islandicus abolishes the oxidative stress-induced expression of a large number of genes. ΔsisperR exhibits enhanced tolerance to H2O2 treatment. Consistently, SisPerR overexpression leads to hypersensitivity to of the cells to the H2O2 treatment. We show that the expression of Dps and MntH which are involved in the regulation of cellular metal ion homeostasis, but not that of ROS scavenging enzymes, is significantly up-regulated with H2O2 treatment and repressed by SisPerR. In summary, this study has established that SisPerR is a repressive redox-sensing transcription factor regulating the intracellular metal ion homeostasis in Saccharolobus islandicus for the oxidative stress defense.
