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REVEILLE5 buffers the activity of CIRCADIAN CLOCK ASSOCIATED1 to modulate thermoresponsive hypocotyl growth in Arabidopsis

ABSTRACT: REVEILLE5 buffers the activity of CIRCADIAN CLOCK ASSOCIATED1 to modulate thermoresponsive hypocotyl growth in Arabidopsis

PROVIDER: PRJNA748032 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15192935_1.fastq.gz	Fastqsanger.gz
	SRR15192935_2.fastq.gz	Fastqsanger.gz
	SRR15192936_1.fastq.gz	Fastqsanger.gz
	SRR15192936_2.fastq.gz	Fastqsanger.gz
	SRR15192937_1.fastq.gz	Fastqsanger.gz

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REVEILLE5 buffers the activity of CIRCADIAN CLOCK ASSOCIATED1 to modulate thermoresponsive hypocotyl growth in Arabidopsis [RNA-seq]

Project description:REVEILLE5 buffers the activity of CIRCADIAN CLOCK ASSOCIATED1 to modulate thermoresponsive hypocotyl growth in Arabidopsis [RNA-seq]

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REVEILLE5 buffers the activity of CIRCADIAN CLOCK ASSOCIATED1 to modulate thermoresponsive hypocotyl growth in Arabidopsis [ChIP-seq]

Project description:REVEILLE5 buffers the activity of CIRCADIAN CLOCK ASSOCIATED1 to modulate thermoresponsive hypocotyl growth in Arabidopsis [ChIP-seq]

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B-box family proteins BBX18 and BBX23 control thermoresponsive hypocotyl growth in Arabidopsis

Project description:Plants coordinate their growth and developmental programs with various endogenous signals and environmental challenges such as seasonal and diurnal temperature fluctuations. The bHLH transcription factor PIF4 plays critical roles in thermoresponsive hypocotyl growth in Arabidopsis, and the evening complex component ELF3 negatively regulates PIF4's activity for downstream gene expression and hypocotyl elongation at elevated temperature. However, how warm temperature signal is transmitted to ELF3 is not known. Here, we report the identification of two B-Box protein BBX18/BBX23 as new regulators of thermomorphogenesis in Arabidopsis. Mutations of BBX18/BBX23 confer reduced thermoresponsive hypocotyl elongation. Overexpression of BBX18 enhances the sensitivity of hypocotyl growth to elevated temperature, which is dependent on the function of PIF4 and RING E3 ligase COP1, respectively. Both BBX18 and BBX23 interact with ELF3 or COP1, relegating the protein abundance of ELF3 at warm temperature. Further, the expression of multiple thermoresponsive genes is impaired in both the PIF4 single mutant and BBX18/BBX23 double mutant. In addition, both the transcription and protein levels of BBX18/BBX23 are up-regulated by elevated ambient temperature. Thus, our findings reveal the important roles of B-Box proteins in plant thermomorphogenesis, and build a new connection from warm temperature information to ELF3 and its downstream signaling components.

2018-12-21 | GSE108162 | GEO

REVEILLE5 buffers the activity of CIRCADIAN CLOCK ASSOCIATED1 to modulate thermoresponsive hypocotyl growth in Arabidopsis

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-12-01 | GSE180382 | GEO

REVEILLE5 buffers the activity of CIRCADIAN CLOCK ASSOCIATED1 to modulate thermoresponsive hypocotyl growth in Arabidopsis [ChIP-seq]

Project description:To further understand how RVE5 controls thermo-responses, we generated RVE5-MYC overexpression plants with the CaMV 35S promoter. Using these RVE5-MYC overexpression plants grown at 29°C, we performed Chromatin Immunoprecipitation-Sequencing (ChIP-Seq).

2022-12-01 | GSE180381 | GEO

REVEILLE5 buffers the activity of CIRCADIAN CLOCK ASSOCIATED1 to modulate thermoresponsive hypocotyl growth in Arabidopsis [RNA-seq]

Project description:To understand how RVE5 regulates thermomorphogenic gene expression, we carried out an RNA-Seq analysis of WT and rve5-2 T-DNA mutant (GK_225C06) treated at 22°C and 29°C.

2022-12-01 | GSE180380 | GEO

ELONGATED HYPOCOTYL 5 mediates blue light signalling to the Arabidopsis circadian clock

Project description:Circadian clocks are gene networks producing 24-h oscillations at the level of clock gene expression that is synchronized to environmental cycles via light signals. The ELONGATED HYPOCOTYL 5 (HY5) transcription factor is a signalling hub acting downstream of several photoreceptors and is a key regulator of photomorphogenesis. Here we describe a mechanism by which light quality could modulate the pace of the circadian clock through controlling abundance of HY5. We show that hy5 mutants display remarkably shorter period rhythms in blue but not in red light or darkness and blue light is more efficient than red to induce accumulation of HY5 at transcriptional and post-transcriptional levels. We demonstrate that the pattern and level of HY5 accumulation modulates its binding to specific promoter elements of majority of clock genes, but only a few of these show altered transcription in the hy5 mutant. Mathematical modelling suggests that the direct effect of HY5 on the apparently non-responsive clock genes could be masked by feed-back from the clock gene network. We conclude that the information on the ratio of blue and red components of the white light spectrum is decoded and relayed to the circadian oscillator, at least partially, by HY5.

2018-09-05 | GSE117797 | GEO

The epidermis coordinates thermoresponsive growth through the phyB-PIF4-auxin pathway

Project description:In plants, an elevation in ambient temperature induces morphological changes including elongation hypocotyls, considered to be adaptive responses to alleviate the heat-induced damages. The high temperature-induced morphological changes are called thermomorphogenesis, which is predominantly regulated by a bHLH transcription factor PIF4. Although PIF4 is expressed in all aerial tissues including the epidermis, mesophyll, and vascular bundle, its tissue-specific functions in thermomorphogenesis are not known. Here, we found that epidermis-specific expression of PIF4 induced constitutive long hypocotyls, while vasculature-specific expression of PIF4 had no effect on hypocotyl growth. Consistently, RNA-Seq and qRT-PCR analyses revealed that auxin responsive genes and growth-related genes were highly activated by epidermal, but not by vascular, PIF4. The epidermal, but not vascular, inactivation of PIF4 by a PIF4 artificial microRNA or a dominant negative form of PIF4 suppressed thermoresponsive gene expression and hypocotyl growth. Additionally, both the block of epidermal auxin signaling and the epidermal overexpression of a thermosensor phytochrome B (phyB) inhibited thermoresponsive growth, indicating that epidermal PIF4-auxin pathway are essential for the temperature responses. We further show that epidermal PIF4 is increased by high temperatures mainly through the transcriptional activation of PIF4. Taken together, our study demonstrates that the epidermis regulates thermoresponsive growth through the phyB-PIF4-auxin pathway.

2020-01-26 | GSE133564 | GEO

B-box family proteins BBX18 and BBX23 control thermoresponsive hypocotyl growth in Arabidopsis

Project description:B-box family proteins BBX18 and BBX23 control thermoresponsive hypocotyl growth in Arabidopsis

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ELONGATED HYPOCOTYL 5 mediates blue light signalling to the Arabidopsis circadian clock

Project description:ELONGATED HYPOCOTYL 5 mediates blue light signalling to the Arabidopsis circadian clock

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