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Rapid 3D Bioprinting of Multicellular Model Recapitulating Pterygium Microenvironment

ABSTRACT: Rapid 3D Bioprinting of Multicellular Model Recapitulating Pterygium Microenvironment

PROVIDER: PRJNA748455 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15204279_1.fastq.gz	Fastqsanger.gz
	SRR15204279_2.fastq.gz	Fastqsanger.gz
	SRR15204280_1.fastq.gz	Fastqsanger.gz
	SRR15204280_2.fastq.gz	Fastqsanger.gz
	SRR15204281_1.fastq.gz	Fastqsanger.gz

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Rapid 3D Bioprinting of Multicellular Model Recapitulating Pterygium Microenvironment

Project description:Pterygium is an ocular surface disorder with high prevalence that can lead to vision impairment. As a pathological outgrowth of conjunctiva, pterygium involves neovascularization and chronic inflammation, but its pathogenesis remains largely unknown. Over the last decade, various types of disease models have been built to study pterygium. Here, we developed a 3D multicellular in vitro pterygium model using the digital light processing (DLP)-based 3D bioprinting of human conjunctival stem cells (hCjSCs). A novel feeder-free culture system was adopted and efficiently expanded the primary hCjSCs with homogeneity, stemness and differentiation potency. The DLP-based 3D bioprinting was able to fabricate hydrogel scaffolds that support the viability and biological integrity of the encapsulated hCjSCs. The bioprinted 3D pterygium model was fabricated with hCjSCs, immune cells and vascular cells to recapitulate the disease microenvironment. Transcriptomic analysis using RNA sequencing (RNA-seq) identified a distinct profile correlated to inflammation response, angiogenesis, and epithelial mesenchymal transition in the bioprinted 3D pterygium model. In addition, the pterygium signatures and disease relevance of the bioprinted model were validated with the public RNA-seq data from patient-derived pterygium tissues. By integrating the stem cell technology and 3D bioprinting, this is the first reported 3D in vitro disease model for pterygium that can be utilized by future studies towards the personalized medicine and the drug screening.

2022-06-01 | GSE180343 | GEO

Gene Expression profiling of pterygium

Project description:Gene Expression profiling of pterygium. Analysis of conjunctiva and pterygium samples. Keywords = pterygium Keywords: repeat sample

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Expression profiling explores pathways involved in pterygium progression

Project description:Pterygium is an ocular surface disease that can cause visual impairment if it progressively invades the cornea. Although many pieces of research showed that ultraviolet radiation triggers pterygium pathological progress, the underlying mechanism in pterygium remains indistinct. In this study, we used microarray to evaluate the changes of transcripts between primary pterygium and adjacent normal conjunctiva samples in China, hoping to find underlying pathways involved in pterygium progression.

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Transcription profiling of pterygium and cornea

Project description:Gene Expression profiling of pterygium (benign growth of the conjunctiva). Analysis of conjunctiva and pterygium samples.

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Project description:Purpose: To characterize the transcriptome of pterygium and to investigate transcriptional regulation of HIF target genes.

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Establishing a reproducible approach to study cellular functions of plants cells with 3D bioprinting

Project description:Capturing cell-to-cell and cell-to-environment signals in a defined 3 dimensional (3D) microenvironment is key to study cellular functions, including cellular reprogramming towards tissue regeneration. A major challenge in current culturing methods is that these methods cannot accurately capture this multicellular 3D microenvironment. In this study, we established the framework of 3D bioprinting with plant cells to study cell viability, cell division, and cell identity. To investigate the cellular reprogramming underlying the initial cell divisions prior to microcallus formation, we collected RNA from isolated differentiated root cells immediately, 1, and 3 days after bioprinting. We showed the cell cycle re-entry of the isolated Arabidopsis and soybean cells leading to the formation of microcalli and of which the timing coincides with the induction of core cell cycle genes and regeneration-related genes. Finally, we showed that the identity of isolated cells of Arabidopsis roots expressing endodermal markers maintained longer periods of time. The framework established in this study paves the way for a general use of 3D bioprinting for studying cellular reprogramming and cell cycle re-entry towards tissue regeneration.

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Project description:Comparison of endogenous gene expression differences between pterygium and conjunctiva tissues RNA from donor-matched pterygium and conjunctiva tissues obtained from four patients were evaluated for differences in gene expression

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Transcriptomics and network analysis highlight potential pathways in the pathogenesis of pterygium

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