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Redundancies in low K signaling of neo-tetraploids leads in increased shoot K [RNA-seq_2]

ABSTRACT: Redundancies in low K signaling of neo-tetraploids leads in increased shoot K [RNA-seq_2]

PROVIDER: PRJNA749717 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15248100.fastq.gz	Fastqsanger.gz
	SRR15248200.fastq.gz	Fastqsanger.gz
	SRR15248300.fastq.gz	Fastqsanger.gz
	SRR15248400.fastq.gz	Fastqsanger.gz
	SRR15248101.fastq.gz	Fastqsanger.gz

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Redundancies in low K signaling of neo-tetraploids leads in increased shoot K [RNA-seq_2]

Project description:Whole Genome Duplication (WGD) events occurred frequently during land plant evolution and their long term benefits to neo-functionalization of genes and speciation is well known (Baduel et al., 2018). However short term benefits, important for the establishment of a new population, are more difficult to observe until Chao et al., 2013 described a fitness advantage under high salinity. Additionally shoot K levels were higher in neo-tetraploids, an important feature since K uptake becomes increasingly more difficult on highly saline soil. The molecular basis for these phenotypes was not known. Here we analyze neo-tetraploid Arabidopsis thaliana plants using RNAseq and ICP-MS to evaluate the effect of mutations and ploidy on the gene expression and shoot ionome. We are able to show that neo-tetraploid plants induce low-potassium (K) signaling to increase their shoot K content. However, we also show that this low-K signaling is distinct form previously studied, externally applied low-K signaling. In this way, we are able to identify new components of the K homeostasis network, which are required to regulate K demand. Additionally we are able to show that while individual components of the K uptake system are not sufficient to increase shoot K, a loss of root hairs abolished the ploidy K phenotype (PPP) as does a defective Casparian strip. Root hairs are the site of entry of K into the root and neo-tetraploids increase their root hair length and density (RHI) to facilitate their higher K demand. The Casparian strip on the other hand enables higher K concentration in the stele of the root, which is required for the increased K content in neo-tetraploids.

2022-07-31 | GSE180818 | GEO

Redundancies in low K signaling of neo-tetraploids leads in increased shoot K [RNA-seq_1]

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Redundancies in low K signaling of neo-tetraploids leads in increased shoot K

Project description:Redundancies in low K signaling of neo-tetraploids leads in increased shoot K

| PRJNA749718 | ENA

Redundancies in low K signaling of neo-tetraploids leads in increased shoot K [RNA-seq_1]

Project description:Redundancies in low K signaling of neo-tetraploids leads in increased shoot K [RNA-seq_1]

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Redundancies in low K signaling of neo-tetraploids leads in increased shoot K

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-07-31 | GSE180819 | GEO

Alteration of TAC1 expression in Prunus species leads to pleiotropic shoot phenotypes

Project description:Alteration of TAC1 expression in Prunus species leads to pleiotropic shoot phenotypes

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HCaRG vs NEO

Project description:Summary: HEK293 cells were transfected with control plasmid (pcDNA1/Neo; Invitrogen) or with the plasmid encoding HCaRG by a standard calcium phosphate co-precipitation method. The clones expressing the highest levels of HCaRG, HCaRG clone 8 and 9 were used in this experiment, while clone transfected with vector alone, Neo clone, served as control. Stable transfectants were synchronized and grown in the presence of 10% FBS for 48 h. Total RNAs were purified with the mini RNeasy kit (Qiagen). Keywords: parallel sample

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Project description:In order to determine the effect of neoR gene in Q175 locus, mRNASeq was performed to compare the transcriptional signatures of zQ175 knock-in neo-in (z_Q175 KI) (CHDI-81003003) and zQ175 KI neo-out (Z_Q175 (neo -) KI) (CHDI-81003019) mouse lines. The z_Q175 KI is a knock-in mouse line with humanized exon 1 (190-200 pure CAG repeats) derived from Q140 KI colony. It contains floxed neo cassette upstream of exon 1. The Z_Q175 KI (neo -) KI was generated by crossing the Z-Q175 KI line with a zp3-cre transgenic line to delete out the neo cassette.

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