Ontology highlight
ABSTRACT:
PROVIDER: PRJNA750271 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR15276055.fastq.gz | Fastqsanger.gz | |||
| SRR15276056.fastq.gz | Fastqsanger.gz | |||
| SRR15276057.fastq.gz | Fastqsanger.gz | |||
| SRR15276058.fastq.gz | Fastqsanger.gz | |||
| SRR15276059.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 27 |
Similar Datasets
Project description:Endophytic bacteria influence plant growth and development and therefore are an attractive resource for applications in agriculture. However, little is known about the impact of these microorganisms on secondary metabolite (SM) production by medicinal plants. Here we assessed, for the first time, the effects of root endophytic bacteria on the modulation of SMs in the medicinal plant Lithospermum officinale (Boraginaceae family), with a focus on the naphthoquinones alkannin/shikonin (A/S). The study was conducted using a newly developed in vitro system as well as in the greenhouse. Targeted and non-targeted metabolomics approaches were used and supported by expression analysis of the gene PGT, encoding a key enzyme in the A/S biosynthesis pathway. Three bacterial strains, Chitinophaga sp. R-73072, Xanthomonas sp. R-73098 and Pseudomonas sp. R-71838 induced a significant increase of diverse SMs, including A/S, in L. officinale in both systems, demonstrating the strength of our approach for screening A/S derivative-inducing bacteria. Our results highlight the impact of root-endophytic bacteria on secondary metabolism in plants and indicate that production of A/S derivatives in planta likely involves cross-modulation of different metabolic pathways that can be manipulated by bacterial endophytes.
2021-08-11 | ST002062 | MetabolomicsWorkbench
Project description:Licorice (Glycyrrhiza uralensis Fisch) flavonoids have many pharmacological effects, as the main chemical component of licorice, its content directly affects the quality of licorice. Methyl jasmine (MeJA) is an important signaling molecule in the secondary metabolic pathway of plants, but the biological mechanisms that stimulating the production of licorice flavonoids and the related changes in transcriptome are still less studied. In this research, the expression of two key enzyme genes: Chalcone synthase (CHS) and Cinnamate 4-hydroxylase (C4H) in the biosynthesis pathway of licorice flavonoids was determined, and it was significantly different after 9 hours of MeJA induction. The transcriptome profiles of licorice cells at 9 hours after MeJA treatment were analyzed to investigate the transcriptional alterations of licorice cell in response to MeJA elicitation by “RNA-seq”. 151, 529 transcripts (200 bp in length) of cDNA from the samples were generated, and 116, 907 unigenes were found. MeJA appeared to stimulate a large number of genes involved in several relevant functional categories, such as carbohydrate metabolism and encoding transcription factors, 11 MYB transcription factors expressed significant differences were screened. This comprehensive description of gene expression information could help elucidate the molecular mechanism of MeJA-mediated biosynthesis of licorice flavonoids and MeJA-regulated network formation.
2019-08-01 | GSE128503 | GEO