Expression data from gastrocnemius muscle of denervated mice ingesting maslinic acid
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PROVIDER: PRJNA750443 | ENA |
REPOSITORIES: ENA
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Project description:Gene expression profiling of the effect of maslinic acid in denervated mice ICR mice were fed either the diet containing 0.27% maslinic acid and the control diet. After 7 days, the sciatic nerve of both legs was cut and a 5-mm piece was excised under anesthesia. Microarray gene expression profiling was conducted for biological replicates of gastrocnemius muscle after 14 days from denervation surgery.
2021-07-29 | GSE181031 | GEO
Project description:We report the application of RNA sequencing technology for transcriptomics analysis of the effects of electrical stimulation on rat denervated gastrocnemius muscle. Muscle samples were selected for gene expression analyses，corresponding to the following categories: Sham operation rats, (SHAM, n = 3), sciatic nerve injury rats (DN, n = 3), and sciatic nerve injury with electrical stimulation (DN-SM, n = 3). A total of 14,334 genes (DEGs) were detected in this study.
2020-07-01 | GSE117440 | GEO
Project description:Transcriptomics analysis of the effects of electrical stimulation on rat denervated gastrocnemius muscle
| PRJNA482119 | ENA
Project description:Samples of denervated GA muscle were compared to contralateral controls to determine transcriptional changes associated with skeletal muscle denervation.
2020-01-06 | GSE62812 | GEO
Project description:Effect of "Sensory Protection" on the transcriptome of Gastrocnemius muscle denervated by Tibial nerve transection in the rat. Comparison is made between the muscle transcriptome in denervated muscle (D), muscle undergoing surgical repair with a motor nerve (immediate repair, or IR) and muscle undergoing surgical repair with a pure sensory nerve (sensory protection, or SP). There are 6 cohorts of rats with 4 to 6 rats in each cohort:<br><br>1 Month Denervated, 1 Month Immediate Repair, 1 Month Sensory Protection, 3 Month Denervated, 3 Month Immediate Repair, 3 Month Sensory Protection. <br><br>For each animal RNA was extracted from both the experimental operated (right) gastrocnemius muscle and the contralateral control unoperated (left) gastrocnemius. Samples were run in duplicate with fluorophor reversal experiments (i.e experimental limb RNA labelled with Cy3 and control limb RNA labelled with Cy5 and vice versa) to control for possible unequal incorporation of the dyes in the reverse transcription reaction.
2009-04-15 | E-MEXP-1580 | biostudies-arrayexpress
Project description:To further analyze the gene expression patterns in denervated muscle atrophy, we have employed whole genome microarray.
2022-06-12 | GSE201025 | GEO
Project description:In skeletal muscle, the pattern of electrical activity regulates the expression of proteins involved in synaptic transmission, contraction and metabolism. Disruptions in electrical activity, resulting from prolonged bed-rest, cast-immobilization or trauma, inevitably lead to muscle atrophy. The mechanisms that regulate muscle atrophy are poorly understood, but it seems likely that changes in gene expression play a key role in initiating and maintaining a muscle atrophy program. Previously, we found that Runx1, a transcription factor previously termed AML1, was substantially induced in muscle following denervation. More recently, we sought to determine whether this increase in Runx1 expression may be causally related to the morphological changes in skeletal muscle that accompany muscle disuse, notably muscle atrophy. We found that Runx1 is indeed required to sustain muscle and to minimize atrophy following denervation. Experiments described here are designed to identify the genes that are regulated by Runx1 in skeletal muscle with the particular goal of identifying genes that regulate muscle atrophy. We propose to use microarray analysis to identify genes, expressed in skeletal muscle, that are mis-regulated in mice lacking Runx1. We inactivated runx1 selectively in skeletal muscle and found that denervated myofibers in mutant mice atrophy far more (90% atrophy) than in wild-type mice (30% atrophy). We therefore reason that Runx1 activates and/or represses genes that are required to sustain muscle and to minimize atrophy. We generated MCK::cre; runx1f/- and runx1f/- control mice. In normal mice, an increase in runx1 expression is detected by two days after denervation and is maximal by five days after denervation. Muscle atrophy is first evident between one and two weeks after denervation. As we wish to avoid detecting global changes in gene expression that are associated with late stages of muscle atrophy, we plan to denervate muscle for three or five days and to compare gene expression in dissected innervated and denervated muscles from mutant and control mice. We will generate thirty samples for comparison-5 replicates per condition: Samples 1-3 from runx1f/- control mice. (1) innervated tibialis anterior muscles (TA); (2) 3-day-denervated TA; (3) 5-day-denervated TA. Samples 4-6 from MCK::cre; runx1f/- mice. (4) innervated TA; (5) 3-day-denervated TA; (6) 5-day-denervated TA. We obtain sufficient total RNA (10 micrograms) from each dissected muscle to avoid pooling samples. We will analyze adult mice of the same age (~six weeks after birth; most will be littermates) and sex-male. It is difficult to anticipate how many genes will be identified in this screen, as few target genes for Runx1 have been identified in any cell type and none in skeletal muscle. Moreover, although we would prefer to focus our attention on genes that are strongly dependent upon Runx1 expression (e.g. more than 5-fold difference in expression in wild-type and mutant mice), we do not know the extent to which target gene expression will depend upon Runx1. For these reasons, in these experiments, we will analyze expression from five âidenticalâ samples, so that we can be confident that even small (e.g. three-fold) differences in expression can be reliably determined. Importantly, in order to confirm results obtained from the microarray data, we will use other assays (RNase protection) to measure RNA expression of candidate genes in innervated and denervated muscles of wild-type and mutant mice.
2008-06-13 | E-GEOD-4411 | biostudies-arrayexpress
Project description:The transcriptome sequencing on muscle tissue from control group (CTX) and denervated group(CTX-DeN). The PI3K/Akt pathway is involved in denervation-aggravated muscle HO.
2024-09-01 | E-MTAB-14258 | biostudies-arrayexpress
Project description:Gastrocnemius muscle-Deletion of TECRL promotes skeletal muscle repair
| PRJNA1067623 | ENA
Project description:In recent years, thanks to the great development of mass spectrometry (MS)-based high-throughput proteomic techniques, a large-scale protein characterization is less challenging. Proteomic approaches have been successfully applied to SM in different experimental models [21–24]; however, to date, there is little information on ECM proteins and how they change during ageing. The identification and quantification of ECM components and their interactions are es-sential steps to understand the role of the matrisome in sarcopenia. In this context, we combined a proteomic approach (i.e., LC-MS/MS and bioinformatic analyses) with mor-phological and morphometrical evaluations at fluorescence and transmission electron microscopy of the gastrocnemius muscle in adult and old mice. This age range has been selected since it has been observed that most of changes in muscle protein expression take place after middle age. The gastrocnemius muscle was selected for analysis since it is prevalently composed of fast-twitch fibre, which are especially affected by atrophy during aging. Our findings highlighted several modifications of ECM protein composition and organization in old mice, which likely play a role in the alteration of muscle properties during aging.
2022-02-17 | PXD027895 | Pride