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Loss of Cdx2 drives direct trophoblast differentiation into trophoblast giant cells via TFAP2C (RNA-Seq)

ABSTRACT: Loss of Cdx2 drives direct trophoblast differentiation into trophoblast giant cells via TFAP2C (RNA-Seq)

PROVIDER: PRJNA750483 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15288939.fastq.gz	Fastqsanger.gz
	SRR15288940.fastq.gz	Fastqsanger.gz
	SRR15288941.fastq.gz	Fastqsanger.gz
	SRR15288942.fastq.gz	Fastqsanger.gz
	SRR15288943.fastq.gz	Fastqsanger.gz

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Loss of Cdx2 drives direct trophoblast differentiation into trophoblast giant cells via TFAP2C

Project description:Loss of Cdx2 drives direct trophoblast differentiation into trophoblast giant cells via TFAP2C

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Loss of Cdx2 drives direct trophoblast differentiation into trophoblast giant cells via TFAP2C (ChIP-Seq)

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Loss of Cdx2 drives direct trophoblast differentiation into trophoblast giant cells via TFAP2C (ATAC-Seq)

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Loss of Cdx2 drives direct trophoblast differentiation into trophoblast giant cells via TFAP2C (RNA-Seq)

Project description:In this experiment, the effect of reduced CDX2 expression on the trophoblast stem cells (TSCs) transcriptome was assayed.

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Loss of Cdx2 drives direct trophoblast differentiation into trophoblast giant cells via TFAP2C (ChIP-Seq)

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Loss of Cdx2 drives direct trophoblast differentiation into trophoblast giant cells via TFAP2C (ATAC-Seq)

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ChIP-seq anlysis of Sox2, Tfap2c, and Cdx2 in trophoblast stem cells.

Project description:To understand the mechanism underlying the transcriptional regulation by Sox2, we analyzed genome-wide binding sites of Sox2, Tfap2c, and Cdx2 in trophoblast stem (TS) cells by chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq). ZHBTc4- and embryo-derived TS cell lines were maintained in the presence of FGF4 and mouse embryonic fibroblasts (MEFs).

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Project description:We characterized the mouse trophoblast giant cell epigenome and gene expression profiles. We then compared these data to our data on underrepresentation in the polyploid trophoblast giant cells. We profiled 5 histone modifications (+ chromatin input) using ChIP-Seq, and digital expression profiles (3' RNA-Seq) for trophoblast giant cells derived from mouse. Furthermore, we profiled digital expression profiles (3' RNA-Seq) for in vivo trophoblast giant cells samples from e9.5 wildtype mouse trophoblast giant cells. We found that underrepresented domains in trophoblast giant cells are enriched for repressive marks and anti-correlate with active marks and transcription.

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