Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Detection of duplication mutations in coding regions in normal lung and lung tumor in wild-type (WT) and FEN1 A159V mutant mice

ABSTRACT: Detection of duplication mutations in coding regions in normal lung and lung tumor in wild-type (WT) and FEN1 A159V mutant mice

PROVIDER: PRJNA750802 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15301095_1.fastq.gz	Fastqsanger.gz
	SRR15301095_2.fastq.gz	Fastqsanger.gz
	SRR15301096_1.fastq.gz	Fastqsanger.gz
	SRR15301096_2.fastq.gz	Fastqsanger.gz
	SRR15301097_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Detection of duplication mutations in coding regions in normal lung and lung tumor in wild-type (WT) and FEN1 A159V mutant mice

Project description:Faithful and efficient Okazaki fragment maturation is the key to maintain the genome integrity. In addition, this process is coupled with deposition of histone proteins. Therefore, functional deficiency in Okazaki fragment maturation may cause duplication mutations. We conduct WES of normal lung and lung tumor from WT and A159V/WT mice to define the duplication mutations caused by defective Okazaki fragment maturation.

2021-11-01 | GSE181154 | GEO

Detection of a genomic duplication by copy number analysis

Project description:Comparative genomic hybridization of strains PN1 (no duplication) and PN1400 (duplication in Chromosome 3)

2018-05-31 | GSE107099 | GEO

Detection of SNCA duplication aritifical mosaics

Project description:To determine sensitivity of a custom 8x60k aCGH design for mosaicism of SNCA gains, a peripheral blood lymphocyte (PBL) DNA sample with a known heterozygous duplication (CN 3, or 50% increase in SNCA) was diluted with a control (wild type, wt) sample (CN 2, screened by MLPA) to create "artifical mosaics" (CN 2.4, 2.3, 2,25, 2.2, 2.15, 2.1). Dilutions, as well as the undiluted heterozygous sample, and the control, were tested, hybridised with Agilent reference DNA.

2016-02-01 | GSE71355 | GEO

Detection of duplication polymorphisms in the Drosophila simulans genome

Project description:In order to characterize duplication polymorphisms in Drosophila simulans, we applied comparative genome hybridization (CGH) using tiling arrays originally designed to cover the full euchromatic genome of its sister species D. melanogaster. We only used the ~900,000 probes in the tiling arrays that had a perfect and unique match to the D. simulans genome (droSim1). We inferred copy number changes with a Hidden Markov Model (HMM) that returned the posterior probabilities for copy number by comparing DNA hybridization intensities between natural isolates. The probabilities of mutation were parsed to make duplication calls. The supplementary file linked to each Sample record contains for each probe, its location in the D. simulans genome and its posterior probability of being duplicated (output from the Hiddem Markov Model)

2011-09-01 | GSE29260 | GEO

Malawi Duplication 20091207

Project description:Malawi Duplication 20091207

| PRJNA120409 | ENA

Drosophila Duplication 20091218

Project description:Drosophila Duplication 20091218

| PRJNA122357 | ENA

ISAba1 mediated intrinsic chromosomal oxacillinase duplication conveys carbapenem resistance

Project description:Tandem duplication of carbapenemase genes amplifies the gene copy number and enhances carbapenem resistance. These amplifications are often heterogenous, transient, locate in plasmids, which often also contribute to heteroresistance. The duplication is especially important for low-hydrolysis activity enzymes, which is often overlooked or even under controversy. Here we reported an intrinsic oxacillinase duplication mediated by two neighbor ISAba1inserted in the same orientation. The duplication is relatively stable, located in chromosome, and the duplication times is up to twenty-five, much larger than previous reports. We provided genomic, transcriptomic, and proteomic evidence that the duplication resulted oxacillinase overproduction and thus contribute to carbapenem resistance. No other possible carbapenem resistance related changes (point mutations of oxaAb, disturbed expression of porin protein and efflux pump) were found during the duplication process. Furthermore, introducing oxaAb flanked by two ISAba1 to A. baumannii via plasmids, mimicked the in vivo duplication process under carbapenem stress. Taken together, ISAba1 mediated duplication of low activity intrinsic antibiotic hydrolysis enzymes could lead to antibiotic resistance for advanced-generation antibiotics.

2023-01-17 | PXD039528 |