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ABSTRACT: WSN/CA04/PR8 infected A549 circRNA

PROVIDER: PRJNA750806 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15305017_1.fastq.gz	Fastqsanger.gz
	SRR15305017_2.fastq.gz	Fastqsanger.gz
	SRR15305018_1.fastq.gz	Fastqsanger.gz
	SRR15305018_2.fastq.gz	Fastqsanger.gz
	SRR15305019_1.fastq.gz	Fastqsanger.gz

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Project description:WSN/CA04/PR8 infected A549 cell line Raw sequence reads

| PRJNA750598 | ENA

The miRNA expression patterns in WSN-infected A549 cells at various time points post-infection.

Project description:To identify miRNAs that are affected by IAV infection, we adopted a systematic approach, and performed a miRNA microarray analysis using a human alveolar adenocarcinoma cell line, A549, which was infected with a common laboratory strain of influenza A/WSN/33(H1N1) virus (WSN). We compared miRNA expression profiles in A549 cells infected with WSN for 2, 6, and 10 hours at a multiplicity of infection (MOI) of 2. Among 955 miRNAs on the microarray, 209 miRNAs were detected in all three infected A549 samples, but when compared with analysis results from mock-infected A549 cells, most expression changes were less than 1.5-fold , which was consistent with a previous report (Buggele et al., 2013). Therefore, we established 1.5-fold expression change as a threshold to select for miRNAs potentially affected by WSN infection. Further screening revealed 116 miRNAs that underwent 1.5-fold or greater expression changes at any timepoint following WSN infection.

2018-05-31 | GSE115069 | GEO

Identification and characterization of circular RNAs in the A549 cells following Influenza A virus infection

Project description:To identify the dysregulated circRNAs expression during IAV infection, A549 cells were inoculated with PR8 (MOI = 2) and harvested at 24 h p.i., while uninfected samples were regarded as controls. Subsequently the high-throughput circRNA microarray was performed (Arraystar Human circRNA Array V2).

2025-01-28 | GSE197125 | GEO

Host cell gene expression in Influenza A/WSN/33 (H1N1) infected A549 cells at 10 hour post infection

Project description:We used the microarray data to analyze host cells response on A549 cells infected with A/WSN/33 (H1N1) The A/WSN/33 (H1N1) infected A549 cells were harvested at 10 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A/WSN/33 (H1N1) infection.

2013-01-03 | E-GEOD-31470 | biostudies-arrayexpress

Host cell gene expression in Influenza A/WSN/33 (H1N1) infected A549 cells at 2, 4 and 6 hours post infection

Project description:We used the microarray data to analyze host cells response on A549 cells infected with A/WSN/33 (H1N1) The A/WSN/33 (H1N1) infected A549 cells were harvested at 2, 4 and 6 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A/WSN/33 (H1N1) infection.

2013-01-03 | E-GEOD-31469 | biostudies-arrayexpress

Host cell gene expression in Influenza A/WSN/33 (H1N1) infected A549 cells at 10 hour post infection

Project description:We used the microarray data to analyze host cells response on A549 cells infected with A/WSN/33 (H1N1)

2013-01-03 | GSE31470 | GEO

Host cell gene expression in Influenza A/WSN/33 (H1N1) infected A549 cells at 2, 4 and 6 hours post infection

Project description:We used the microarray data to analyze host cells response on A549 cells infected with A/WSN/33 (H1N1)

2013-01-03 | GSE31469 | GEO

C646, a Novel p300/CREB-Binding Protein-Specific Inhibitor of Histone Acetyltransferase, Attenuates Influenza A Virus Infection

Project description:Whole-genome data was developed from influenza virus infected A549 cells to better characterize the effect of C646 on influenza virus infection A549 cells were treated with C646 or DMSO for 10 h, and then were infected with A/WSN/33 virus (WSN; H1N1) at a multiplicity of infection (MOI) 2. A549 cells for microarray studies were collected at different times. The gene expression in A549 cells was compared between C646-treated group and DMSO-treated group.

2016-01-07 | E-GEOD-72503 | biostudies-arrayexpress

The interaction of influenza A NS1 and cellular TRBP protein modulates the function of RNA interference machinery

Project description:Influenza A virus (IAV), one of the most prevalent respiratory diseases, causes pandemics around the world. The multifunctional non-structural protein 1 (NS1) of IAV is a viral antagonist that suppresses host antiviral response. However, the mechanism by which NS1 modulates the RNA interference (RNAi) pathway remains unclear. Here, we identified interactions between NS1 proteins of Influenza A/PR8/34 (H1N1; IAV-PR8) and Influenza A/WSN/1/33 (H1N1; IAV-WSN) and Dicer’s cofactor TAR-RNA binding protein (TRBP). We found that the N-terminal RNA binding domain (RBD) of NS1 and the first two domains of TRBP protein mediated this interaction. Furthermore, two amino acid residues (Arg at position 38 and Lys at position 41) in NS1 were essential for the interaction. We generated TRBP knockout cells and found that NS1 instead of NS1 mutants (two-point mutations within NS1, R38A/K41A) inhibited the process of microRNA (miRNA) maturation by binding with TRBP. PR8-infected cells showed masking of short hairpin RNA (shRNA)-mediated RNAi, which was not observed after mutant virus-containing NS1 mutation (R38A/K41A, termed PR8/3841) infection. Moreover, abundant viral small interfering RNAs (vsiRNAs) were detected in vitro and in vivo upon PR8/3841 infection. We identify, for the first time, the interaction between NS1 and TRBP that affects host RNAi machinery.

2022-04-15 | GSE189776 | GEO

Immune response of TRIM28 KO cells infected with H1N1 IAV

Project description:RNAseq analysis of A549-TRIM28 KO cells that were infected with low pathogenic laboratory strain PR8 (H1N1) Differentially expressed genes in infected vs. non-infected cells Project: A549_TRIM28_KO

2022-08-31 | GSE206188 | GEO

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