Project description:We report on P7 dental pulp root and crown genetics and pathways altered by the deletion of Tgfbr2 in the dental pulp and odontoblast cells to investigate the short tooth root phenotype demonstrated in the Tgfbr2 conditional knockout mice.
Project description:Objectives: Dental pulp stem cells are crucial in immune response regulation. However, sub-clusters of these cells involved in deep caries progression remain unidentified. This study explores the role of Intercellular adhesion molecule 1-positive dental pulp stem cells (ICAM1+DPSCs) within the immune microenvironment of dental pulp tissue affected by deep caries, aiming to establish a theoretical foundation and novel strategies for its prevention and treatment.Methods: Use flow cytometry to sort ICAM1+ DPSCs and ICAM1- DPSCs for RNA-seq.
Project description:Dental pulp plays a crucial role for dental health, and dental pulp aging influences their regenerative and reparative function. However, the underlying molecular mechanisms of dental pulp aging are not exhaustively understood, and thereby an in-depth and complete understanding of the aged dental pulp is of foremost importance. This study aimed to explore the heterogeneity of young and aged dental pulp tissue using single-cell RNA sequencing (scRNA-seq).
Project description:Dental pulp cells obtained from several donors proliferated actively in a serum-free medium STK2. The growth rate of dental pulp cells from most donors was higher in the serum-free medium than that in a medium containing 10% serum. DNA microarray analyses showed that gene expression profile of dental pulp cells grown in the serum-free medium was similar to that of cells grown in a medium containing 10% serum. However, several genes related to cell proliferation were up-regulated in dental pulp cells grown in the serum-free medium.
Project description:Transcriptional response of rat dental pulp cells (DPCs) cultured with SAHA at early and late mineralisation time points Transcript profiling of DPC identified several novel genes expression induced and supressed by HDACi at 24 hrs and 14 days under mineralising conditions. SAHA induces several members of the MMP family of endopepsidases (TIMP-1, MMP-9, MMP-13) and other members of the endochondral ossification pathway at 24 h. 8 experiemental parameters were analysed, each carried out in quadruplicate