Training-associated alterations in equine respiratory immunity using RNAseq
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA751079 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR15308349_1.fastq.gz | Fastqsanger.gz | |||
| SRR15308349_2.fastq.gz | Fastqsanger.gz | |||
| SRR15308350_1.fastq.gz | Fastqsanger.gz | |||
| SRR15308350_2.fastq.gz | Fastqsanger.gz | |||
| SRR15308351_1.fastq.gz | Fastqsanger.gz |
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Project description:Neutrophilic airway inflammation is highly prevalent in racehorses in training, with the term mild to moderate equine asthma (MMEA) being applied to the majority of such cases. The current study is largely derived from the strong association between MMEA in racehorses and their entry into a race training program; this has led to our primary aim of measuring the effect of race training on pulmonary immune cell function. The objectives of this study are to characterise the effect of training on the local pulmonary immune system and quantify the magnitude of effect by defining the gene expression of tracheal wash (TW) derived cells from Thoroughbred racehorses prior to (T0) and following commencement of race training (T1). This study demonstrated TW samples to represent a rich source of airway cells and RNA to study airway immunity in the horse and highlighted the benefits of advanced-omic methodological approach to studying the dynamics of equine airway immunity. Intense training induced quantifiable alterations in both gene expression of airway derived cells consistent with deregulation of airway immunity and haemopoietic abnormalities. Respectively, these findings likely reflect the known associations between race training and both airway inflammation and bleeding, in particular offering further insight into the potential mechanisms which underpin training associated airway inflammation.
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Project description:Neutrophilic airway inflammation is highly prevalent in racehorses in training, with the term mild to moderate equine asthma (MMEA) being applied to the majority of such cases. Our proposed study is largely derived from the strong association between MMEA in racehorses and their entry into a race training program. The objectives of this study are to characterise the effect of training on the local pulmonary immune system by defining the gene and protein expression of tracheal wash (TW) derived samples from Thoroughbred racehorses prior to and following commencement of race training. Multiomics analysis detected 2138 differentially expressed genes and 260 proteins during the training period. Gene and protein sets were enriched for biological processes related to acute phase response, oxidative stress, haemopoietic processes, as well as to immune response and inflammation. This study demonstrated TW samples to represent a rich source of airway cells, protein and RNA to study airway immunity in the horse and highlighted the benefits of a multiomics methodological approach to studying the dynamics of equine airway immunity. Findings likely reflect the known associations between race-training and both airway inflammation and bleeding, offering further insight into the potential mechanisms which underpin training associated airway inflammation.
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Project description:Digital gene expression profiling was used to characterize the assembly of genes expressed in equine skeletal muscle and to identify the subset of genes that were differentially expressed following a ten month period of exercise training. The study cohort comprised 7 thoroughbred racehorses from a single training yard. Skeletal muscle biopsies were collected at rest from the gluteus medius at two time points: T1 (unconditioned), (9 +/- 0.5 months old) and T2 (conditioned) (20 +/- 0.7 months old). The most highly abundant genes in the muscle transcriptome were those involved in muscle contraction, aerobic respiration and mitochondrial function. A previously unreported over-representation of genes relating to RNA processing, the stress response and proteolysis was observed. Following training 92 tags were differentially expressed of which 74 were annotated. Sixteen genes showed increased expression, including the mitochondrial genes, ACADVL, MRPS21 and SLC25A29. Among the 58 genes with deceased expression MSTN, a negative regulator of muscle growth had the greatest decrease. Functional analysis of all expressed genes using FatiScan revealed an asymmetric distribution of 482 Gene Ontology groups and 18 KEGG pathways. Functional groups with highly significantly (P < 0.0001) increased expression included mitochondrion, oxidative phosphorylation and fatty acid metabolism while functional groups with decreased expression were mainly associated with structural genes and included the sarcoplasm, laminin complex and cytoskeleton. Examination of muscle expression changes in 7 thoroughbred horses following 10 months of exercise training using digital gene expression with NlaIII.
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