Project description:We present a label free proteome dataset of the vascular sap proteome of three commercially important Eucalyptus species (Eucalyptus camaldulensis, Eucalyptus grandis and Eucalyptus urophylla). Protein extraction from the vascular system was carried out using a pressure bomb, in solution digested and peptides were analyzed using a Q-Exactive instrument. Protein identification was carried out using stringent database searches and only in silico predicted extracellular proteins were considered as part of the sap proteome. The results here described can be used as a reference for the proteome sap analysis of Eucalyptus plants grown under different conditions.
Project description:Chloroplast is a subcellular compartment in which solar radiation is used to generate simple sugar compounds in a complex process named photosynthesis. It is very sensitive to environmental fluctuations and your development is intimately related with the development of the whole plant at different stages. Seasonal climate variations can also drive plant growth and development, favoring a metabolic adaptation of the life cycle with changes in the environment. The characterization of chloroplast proteome dynamics can contribute to a better understanding about the mechanisms involved in leaf development process and seasonal variations in this compartment. Therefore, the goal of this studied was to carried out an exploratory analysis of chloroplast alterations during leaf development and seasonal variations in Eucalyptus grandis chloroplast proteome.
Project description:New RNA-seq data was generated to create a large-scale Seminavis robusta gene expression atlas profiling a wide-range of experimental conditions including life cycle stages and abiotic stressors. These stresses encompass among others changes in temperature and salt concentration, silica depletion, high light exposure, H202 and decadienal treatment.