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MRNA sequencing of SKMEL28 cells

ABSTRACT: mRNA sequencing of SKMEL28 cells

PROVIDER: PRJNA752118 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR15346704_1.fastq.gz	Fastqsanger.gz
	SRR15346704_2.fastq.gz	Fastqsanger.gz
	SRR15346705_1.fastq.gz	Fastqsanger.gz
	SRR15346705_2.fastq.gz	Fastqsanger.gz
	SRR15346706_1.fastq.gz	Fastqsanger.gz

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mRNA sequencing of SKMEL28 cells

Project description:A RNA-seq analysis was performed to characterize the molecular intermediates responsible for the deleterious effects upon DUSP4 loss in SKMEL28 cells. Methods:SKMEL28 cells were transfected with siRNA against DUSP4 or non-targeting control with or without Trametinib (Tram) at 0.25nM. After 16 hours, cell were collected and RNAseq experiments were performed. Results: the expression of MITF and some of its target genes were significantly downregulated in the absence of DUSP4 and completely rescued by trametinib treatment. Conclusions: In mutant melanoma cells, the expression of MITF and its related target genes is subject to regulation by the DUSP4-ERK axis.

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