Project description:We looked for difference in genetic expression in 4 cell lines that have the same genetic background but reproduce breast cancer progression. Especially, we identied genes that were overexpressed in late cancer progression (MCFT1 and MCFCA1) but not in early stages (MCF10A and MCFNeoT).
Project description:We report the role of AIB1D4 in early stage breast cancer progression. Using CRISPR editing, we generated isogeneic cell lines that only express AIB1D4 and not full length AIB1 then extracted RNA for sequencing from cell lines and mamary fat pad xenografts in SCID/Beige mice. Data was aligned to Hg19 with STAR and processed with EdgeR.
Project description:In order to identify novel molecular targets associated with TNBC progression, we initially performed transcriptome analysis using RNA sequencing in breast cancer cell lines, classified as either the luminal subtype (MCF-7, T47D, ZR-75B) or basal-like subtype (MDA-MB-231, MDA-MB-435, Hs578T).
Project description:TMT16 plex MS proteomics based comparison of 8 breast cancer cell lines in duplicates. Peptides were fractionated by HiRIEF and analyzed by Q-exactive HF.
The cell lines are: HCC1937
MDAMB453
SUM185PE
MDAMB468
BT549
HCC1599
HCC2157
MDAMB231