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B cells after CD74 activation.

ABSTRACT: B cells after CD74 activation.

PROVIDER: PRJNA752998 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15372671_1.fastq.gz	Fastqsanger.gz
	SRR15372671_2.fastq.gz	Fastqsanger.gz
	SRR15372672_1.fastq.gz	Fastqsanger.gz
	SRR15372672_2.fastq.gz	Fastqsanger.gz
	SRR15372673_1.fastq.gz	Fastqsanger.gz

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Genome wide H3K4me2 detection after CD74 activation.

Project description:CD74 (invariant chain), expressed on B cells, is directly involved in shaping the B cell repertoire by regulating their survival in health and disease. Binding of its ligand, macrophage migration inhibitory factor (MIF), induces a cascade that results in CD74 intramembrane proteolysis, and the release of the CD74 intracellular domain (CD74-ICD). CD74-ICD translocates to the nucleus where it induces activation of transcription. In the current study, we characterized the transcription factor activity of CD74-ICD. Following histone modifications we are able to characterize CD74's binding sites as regulatory areas.

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Genome wide H3K4me2 detection after CD74 activation.

Project description:Genome wide H3K4me2 detection after CD74 activation.

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CD74 role in transcription regulation [ChIP-seq]

Project description:CD74 activation was previously shown to affect gene transcription. The goal of this study was to research its role as a transcriptor regulator. CD74 was activated in primary CLL cells obtained from patients for 1 h. The cells were then fixed using DSG and formaldhyde, and ChIP-seq was conducted.

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CD74 downregulation in placental macrophages and its influence on trophoblast cells.

Project description:Preeclampsia is a disease of pregnant women, which is characterized by hypertension, proteinuria and chronic inflammation. There is a growing body of evidence that cause of preeclampsia lies within immunological aspect of pregnancy. This study aimed to analyze the role of CD74 in preeclampsia with a focus on its influence on communication between placental macrophages (Hofbauer cells) and trophoblasts. We have found CD74 to be highly dysregulated in preeclamptic placenta by real-time RT-PCR and Western blot methods. We identified Hofbauer cells to express the highest levels of CD74 in placenta by immunofluorescence and flow cytometry and that CD74 in preeclamptic Hofbauer cells is lower than in controls. We have performed a transcriptome analysis on human blood monocyte-derived macrophages that were non- or IL-4-activated and treated with small interfering RNA against CD74 (siRNA CD74) or non-targeting siRNA (siRNA non-targeting) as control.

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Elucidate the role of CD74 in gene transcription regulation by analyzing the genes up or down regulated after 8 hours of activation in human naive B cells.

Project description:CD74 (invariant chain), expressed on B cells, is directly involved in shaping the B cell repertoire by regulating their survival in health and disease. Binding of its ligand, macrophage migration inhibitory factor (MIF), induces a cascade that results in CD74 intramembrane proteolysis, and the release of the CD74 intracellular domain (CD74-ICD). CD74-ICD translocates to the nucleus where it induces activation of transcription. In the current study, we characterize the transcription regulator activities of CD74 by analysing its ability to alter gene expression in mRNA levels.

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Project description:CD74 activation was previously shown to affect gene transcription. The goal of this study was to reasarch its role as a transcriptor regulator. CD74 was activated in primary CLL cells obtained from patients for 2 and 8 h. mRNA was then extracted, and mRNA-seq was conducted.

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The role of CD74 in Hematopoietic Stem Cells

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Project description:The influence of CD74 expression on the HLA peptidome of the H1 melanoma cell line was analyzed by label-free quantitation mass spectrometry of HLA ligand extracts obtained from CD74 siRNA & control siRNA treated cells.

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