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Transcription-wide distribution of dihydrouridine (D) into mRNAs reveals its requirement for meiotic chromosome segregation [Ribo/RNA-seq]

ABSTRACT: Transcription-wide distribution of dihydrouridine (D) into mRNAs reveals its requirement for meiotic chromosome segregation [Ribo/RNA-seq]

PROVIDER: PRJNA753526 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15410423_1.fastq.gz	Fastqsanger.gz
	SRR15410423_2.fastq.gz	Fastqsanger.gz
	SRR15410424_1.fastq.gz	Fastqsanger.gz
	SRR15410424_2.fastq.gz	Fastqsanger.gz
	SRR15410425_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Transcription-wide distribution of dihydrouridine (D) into mRNAs reveals its requirement for meiotic chromosome segregation [Ribo/RNA-seq]

Project description:Here we have developed Rho-seq, an integrated pipeline detecting a range of modifications through differential modification-dependent Rhodamine labeling. Using Rho-seq, we report that the reduction of uridine to dihydrouridine by the Dus reductase family targets tRNAs in E. coli but expands to mRNAs is yeast. The modified mRNAs are enriched for cytoskeleton related encoded protein. We show that the a-tubulin encoding mRNA nda2 undergoes dihydrouridination, which affects its protein expression level. The absence of the modification onto the nda2 mRNA strongly impacts meiosis by inducing a metaphase delay or by completely preventing the formation of spindles during meiosis I and meiosis II, eventually resulting in low gamete viability. Collectively these data show that the codon specific reduction of uridine within mRNA is required for proper meiotic chromosome segregation and gamete viability.

2021-10-26 | GSE181824 | GEO

Transcription-wide distribution of dihydrouridine (D) into mRNAs reveals its requirement for meiotic chromosome segregation.

Project description:Transcription-wide distribution of dihydrouridine (D) into mRNAs reveals its requirement for meiotic chromosome segregation.

| PRJNA607947 | ENA

Transcription-wide distribution of dihydrouridine (D) into mRNAs reveals its requirement for meiotic chromosome segregation. [RNA-Seq]

2021-10-26 | GSE145683 | GEO

Transcription-wide distribution of dihydrouridine (D) into mRNAs reveals its requirement for meiotic chromosome segregation. [Rho-Seq]

2021-10-26 | GSE145685 | GEO

Transcription-wide distribution of dihydrouridine (D) into mRNAs reveals its requirement for meiotic chromosome segregation. [RNA-Seq]

Project description:Transcription-wide distribution of dihydrouridine (D) into mRNAs reveals its requirement for meiotic chromosome segregation. [RNA-Seq]

| PRJNA607952 | ENA

Transcription-wide distribution of dihydrouridine (D) into mRNAs reveals its requirement for meiotic chromosome segregation. [Rho-Seq]

Project description:Transcription-wide distribution of dihydrouridine (D) into mRNAs reveals its requirement for meiotic chromosome segregation. [Rho-Seq]

| PRJNA607960 | ENA

Transcription-wide distribution of dihydrouridine (D) into mRNAs reveals its requirement for meiotic chromosome segregation.

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-10-26 | GSE145686 | GEO

Transcriptome-wide mapping of dihydrouridine sites with D-seq

Project description:We report the development of D-Seq, a sequencing based technique for identification of dihydrouridine (D) modifications within RNA. We performed D-Seq on S. cerevisiae and find the first evidence for dihydrouridylation of endogenous mRNAs.

2022-04-16 | GSE175549 | GEO

Centromere Pairing Enables Correct Segregation of Meiotic Chromosomes

Project description:Centromere Pairing Enables Correct Segregation of Meiotic Chromosomes

| PRJNA1069033 | ENA

Differential requirement for Bub1 and Bub3 in regulation of meiotic versus mitotic chromosome segregation.

Project description:Accurate chromosome segregation depends on the proper attachment of kinetochores to spindle microtubules before anaphase onset. The Ipl1/Aurora B kinase corrects improper attachments by phosphorylating kinetochore components and so releasing aberrant kinetochore-microtubule interactions. The localization of Ipl1 to kinetochores in budding yeast depends upon multiple pathways, including the Bub1-Bub3 pathway. We show here that in meiosis, Bub3 is crucial for correction of attachment errors. Depletion of Bub3 results in reduced levels of kinetochore-localized Ipl1 and concomitant massive chromosome missegregation caused by incorrect chromosome-spindle attachments. Depletion of Bub3 also results in shorter metaphase I and metaphase II due to premature localization of protein phosphatase 1 (PP1) to kinetochores, which antagonizes Ipl1-mediated phosphorylation. We propose a new role for the Bub1-Bub3 pathway in maintaining the balance between kinetochore localization of Ipl1 and PP1, a balance that is essential for accurate meiotic chromosome segregation and timely anaphase onset.

| S-EPMC7147105 | biostudies-literature

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