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AZA+atRA reprogramming of head and neck squamous cell carcinoma cell line T-HEp3

ABSTRACT: AZA+atRA reprogramming of head and neck squamous cell carcinoma cell line T-HEp3

PROVIDER: PRJNA753542 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR15411300_1.fastq.gz	Fastqsanger.gz
	SRR15411300_2.fastq.gz	Fastqsanger.gz
	SRR15411301_1.fastq.gz	Fastqsanger.gz
	SRR15411301_2.fastq.gz	Fastqsanger.gz
	SRR15411302_1.fastq.gz	Fastqsanger.gz

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AZA+atRA reprogramming of head and neck squamous cell carcinoma cell line T-HEp3

Project description:We report the RNA expression profile in the AZA+atRA reprogrammed tumorigenic (T-HEp3) HNSCC cell line. T-HEp3 is a human epidermoid carcinoma, which grows on chorioallantoic membrane and metastasizes with high efficiency into the organs of chick embryo. Upon serial passage for more than 40 times in vitro, T-HEp3 loses tumorigenic and metastatic potential and termed as dormant D-HEp3. This is an ideal cell line model system to study cancer dormancy.

2023-04-27 | GSE181837 | GEO

AZA+atRA reprogramming of head and neck squamous cell carcinoma cell line T-HEp3, and enhancer analysis by H3K27ac ChIP-seq in HNSCC T-HEP3 and D-HEp3 cell lines

Project description:AZA+atRA reprogramming of head and neck squamous cell carcinoma cell line T-HEp3, and enhancer analysis by H3K27ac ChIP-seq in HNSCC T-HEP3 and D-HEp3 cell lines

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macroH2A2 overexpression in head and neck squamous cell carcinoma cell line T-HEp3.

Project description:We report the RNA expression profile in the restoration of macroH2A2 in tumorigenic (T-HEp3) HNSCC cell line. T-HEp3 is a human epidermoid carcinoma, which grows on chorioallantoic membreane and metastasizes with high efficency into the organs of chick embryo. Upon serial paasage for more than 50 times in vitro , T-HEp3 loses tumorigenic and metastatic potential and termed as dormant D-HEp3. This is an ideal cell line model system to study cancer dormancy.

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Differential RNA expression in human neck and squamous cell carcinoma cell lines (T-HEp3 and D-HEp3).

Project description:We report the RNA expression profile in the tumorigenic (T-HEp3) and dormant (D-HEp3) HNSCC cell lines. T-HEp3 is a human epidermoid carcinoma, which grows on chorioallantoic membreane and metastasizes with high efficency into the organs of chick embryo. Upon serial paasage for more than 50 times in vitro , T-HEp3 loses tumorigenic and metastatic potential and termed as D-HEp3. This is an ideal cell line model system to study cancer dormancy.

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macroH2A2 overexpression in head and neck squamous cell carcinoma cell line T-HEp3.

Project description:macroH2A2 overexpression in head and neck squamous cell carcinoma cell line T-HEp3.

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RNA-seq of head and neck cancer cell lines

Project description:Transcriptome analysis in head and neck cancer cell lines, FaDu and UMSCC47 with and without 5-aza'2-deoxycytidine(Aza)/trichostatin A(TSA) treatment.

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Differential RNA expression in human neck and squamous cell carcinoma cell lines (T-HEp3 and D-HEp3).

Project description:Differential RNA expression in human neck and squamous cell carcinoma cell lines (T-HEp3 and D-HEp3).

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Expression profiling of proliferative T-HEp3 and dormant D-HEp3 HNSCC cells in vivo

Project description:The objective of this study was to obtain expression profiles of proliferative T-HEp3-GFP and dormant D-HEp3-GFP cells after one week in vivo. The second objective was find tumor cells quiescence associated genes in dormant D-HEp3 cells that are only quiescent when injected in vivo. In this case we compared cells one week growing vs. dormant for the indicated cells in chick embryo CAMs. After one week 5 embryos per cell line carrying the indicated cells were isolated, tumors collagenased as described below and sorted for GFP-high cells usig a MoFlo machine. The sorted cells > 5x10^4 were used to extract RNA and the pure RNA was used to perform expression profiling using the Affymetrix HG-u133plus2 arrays. Because of the low amount of D-HEp3 (dormant) cells recovered all tumor cells from the dormant nodules were pooled. The same was done for proliferative-sorted T-HEp3-GFP cells to allow comparisons. Arrays were performed in triplicate. 5 T-HEp3-GFP tumors and 5 D-HEp3-GFP nodules were processed for preparation of single cells suspensions, RNA extracted and then pools of T-HEp3-GFP and D-HEp3-GFP sorted cells were analyzed in triplicate arrays

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Enhancer analysis by H3K27ac ChIP-seq in HNSCC T-HEP3 and D-HEp3 cell lines

Project description:Identification of active and differentially enriched enhancers in T-HEp3 and D-HEp3 cell lines.

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AZA+atRA reprogramming of head and neck squamous cell carcinoma cell line T-HEp3, and enhancer analysis by H3K27ac ChIP-seq in HNSCC T-HEP3 and D-HEp3 cell lines

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-04-27 | GSE181839 | GEO