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ABSTRACT: Genome sequencing of cellulase producing fungi Penicillium oxalicum

PROVIDER: PRJNA754273 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15440698_1.fastq.gz	Fastqsanger.gz
	SRR15440699_1.fastq.gz	Fastqsanger.gz
	SRR15440699_2.fastq.gz	Fastqsanger.gz

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Comparative genomic, transcriptomic and secretomic profilings of Penicillium oxalicum HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106, and identification of two novel regulators for cellulase and xylanase gene expression

Project description:In this study,comparative genomic, transcriptomic and secretomic profilings of Penicillium oxalicum HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106 were employed to screen for novel regulators for cellulase and xylanase gene expression.

2016-08-31 | GSE80076 | GEO

Large scale transcriptome analysis was used to identify the genes most related to the transcription of cellulase genes and/or xylanase genes expression

Project description:Based on the transcriptome data of deletion mutants of different genes and different culture conditions of Penicillium oxalicum, the correlation of gene transcription levels in the whole genome was calculated to explore the genes most related to the transcription of cellulase gene and/or xylanase gene in Penicillium oxalicum, including transcription factors, sugar transporters and other functional genes.

2023-07-22 | GSE155790 | GEO

Comparative DIA-proteomics of cultures from P. oxalicum strains dataPoxTMA7 and WT

Project description:In the previous works, we have identified a microprotein PoxTMA7 positively regulate cellulase and xylanase production in Penicillium. oxalicum. Intergrated Ribo-seq and RNA-seq analysis demonstrated that the microprotein PoxTMA7 reprogram translation landscape in P. oxalicum. Particularly, the deletion of the gene PoxTMA7 leads to the reduced translation efficiency (TE) of key cellulase and xylanase genes. To evaluated the effects of PoxTMA7 on the abundance of cellulase, xylanase and other extracellular proteins in P. oxalicum. We performed comparative DIA-proteomics of cultures from P. oxalicum strains dataPoxTMA7 and WT.

2026-05-28 | PXD078922 | Pride

Transcription factor CrzA modulate fungal development and cellulolytic gene expression in Penicillium oxalicum

Project description:The goals of this study are to compare different gene expressions for Penicillium oxalicum wild type strain (WT), CrzA deletion strain (ΔCrzA) and CrzA recover strain (ReCrzA) in 2% starch as carbon sources. The correlation analysis results between the various samples showed that the gene expression levels of the wild strain and the RecrzA strain were similar, and the gene expression levels between ΔcrzA and the wild strain were significantly different. The deletion of CrzA significantly down-regulates the expression levels of conidia pigment synthesis genes, and spore wall synthesis-related genes of Penicillium oxalicum, and also has a regulatory effect on the expression levels of genes related to the sporulation process. And the absence of CrzA can broadly down-regulate the expression level of cellulase-encoding genes, indicating that CrzA can cause a decrease in cellulase synthesis by affecting the expression of cellulase-encoding genes. The information provided by this study indicates that CrzA function is necessary for the mycelial development and cellulase activity of Penicillium oxalicum.

2021-05-29 | GSE175771 | GEO

Effects of transcription factor POX04513 on the transcription of cellulase genes and xylanase genes in Penicillium oxalicum

Project description:To investigate the effect of the transcription factor POX04513 in Penicillium oxalicum on the expression levels of cellulase and xylanase genes in solid and liquid fermentation

2023-05-06 | GSE154882 | GEO

The effects of ClrB internal mutant overexpression and creA deletion on the transcriptome of Penicillium oxalicum

Project description:The synthesis of cellulolytic enzymes by filamentous fungi are mainly regulated at the transcriptional level. The essential role of transcriptional activator ClrB/CLR-2 in the induction of cellulase genes has been reported in several fungal species. In this study, we identified the C-terminal region of ClrB in Penicillium oxalicum as a transcriptional activation domain using a yeast reporter gene system. Overexpression of a mutant ClrBID fusing the DNA-binding and transcriptional activation domains of ClrB together enabled cellulase production under cellulase non-inducing conditions. Strikingly, the ClrBID-overexpression strain produced cellulase in the presence of glucose at a level similar to that of reference strain on cellulose. Combination of ClrBID overexpression and deletion of carbon catabolite-repressor gene creA suggested an additive effect of the two manipulations on carbon catabolite de-repression. A similar modification of ClrB in Aspergillus niger also resulted in cellulase production on glucose. The results indicated that the post-transcriptional control of ClrB activity involves its central region, and provided a potential strategy for engineering Penicillium and Aspergillus strains for cellulase production on easy-to-use carbon sources.

2019-01-01 | GSE120416 | GEO

Comparative proteomics profiling of Penicillium oxalicum under induction and repression of cellulase biosynthesis

Project description:Compararive analysis of Penicillium oxalicum HP7-1 after cultured in medium containing 1% glucose and 1% Avicel respectively, in order to explaining the mechanism of cellulase and xylanase production at protein level. Identify the small proteins encoded by the predicted sORFs based on the genomic analysis using mass spectrometry and analyze their expression under cellulose-induction and non-induction conditions.

2026-05-28 | PXD073955 | Pride

Transcriptomic analysis of cellulolytic fungus Penicillium oxalicum and its sporogenesis related genes deletion strains

Project description:RNA-seq was used to compare differential gene expressions for Penicillium oxalicum wild type strain (M12) and sporognesis related genes knock out strains.The goals of this study are to construct the sporogenesis regulation pathway of Penicillium oxalicum. Examination of differential gene expressions by digital gene expression tag profiling in Penicillium oxalicum wild type strain and nine mutant strains (flbA knoutout strain, flbB knoutout strain, flbC knoutout strain, flbD knoutout strain, flbE knoutout strain,wetA knoutout strain, abaA knoutout strain,stuA knoutout strain, swi6 knoutout strain)

2015-09-01 | E-GEOD-72523 | biostudies-arrayexpress

Fif3 regulates the development of Penicillium oxalicum fungi and the expression of cellulolytic genes

Project description:The goals of this study are to compare different gene expressions for Penicillium oxalicum wild type strain (WT) and Fif3 deletion strain (DFif3) under conditions of 1% bran + 1% microcrystalline cellulose as carbon source. The results of correlation analysis between each sample showed that the gene expression level of DFif3 was significantly different from that of the WT strain. The deletion of DFif3 significantly down-regulates the expression levels of sporulation process genes. And the absence of DFif3 can broadly up-regulate the expression level of cellulase-encoding genes, indicating that DFif3 can cause a decrease in cellulase synthesis by affecting the expression of cellulase-encoding genes.

2024-03-13 | GSE261111 | GEO

The F-box protein Fbx23 regulates the development of Penicillium oxalicum fungi and the expression of cellulolytic genes

Project description:The goals of this study are to compare different gene expressions for Penicillium oxalicum wild type strain (WT), Fbx23 deletion strain (Δfbx23), and Fbx23 ecover strain (Refbx23) under conditions of 2% glucose or 1% bran + 1% microcrystalline cellulose as carbon source. The results of correlation analysis between each sample showed that the gene expression level of Δfbx23 was significantly different from that of the WT strain. The deletion of Fbx23 significantly down-regulates the expression levels of sporulation process genes. And the absence of Fbx23 can broadly up-regulate the expression level of cellulase-encoding genes, indicating that Fbx23 can cause a decrease in cellulase synthesis by affecting the expression of cellulase-encoding genes.

2023-04-03 | GSE228483 | GEO

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