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Effects of BPA, BPAF, and BPS on Endochondral Ossification and on the Transcriptome of Ex Vivo Murine Limb Bud Cultures II

ABSTRACT: Effects of BPA, BPAF, and BPS on Endochondral Ossification and on the Transcriptome of Ex Vivo Murine Limb Bud Cultures II

PROVIDER: PRJNA754363 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15441800_1.fastq.gz	Fastqsanger.gz
	SRR15441800_2.fastq.gz	Fastqsanger.gz
	SRR15441801_1.fastq.gz	Fastqsanger.gz
	SRR15441801_2.fastq.gz	Fastqsanger.gz
	SRR15441802_1.fastq.gz	Fastqsanger.gz

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Effects of BPA, BPAF, and BPS on Endochondral Ossification and on the Transcriptome of Ex Vivo Murine Limb Bud Cultures

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Effects of BPA, BPF and BPS treatments on mESC EB-based global and neural differentiations.

Project description:Purpose: the goal of this study is to determine the changes in gene expression during EB differentiation after BPA, BPF and BPS treatments, as indications of potential developmental toxicity. Methods: Transcriptomics profiling for EB samples treated with 100 nM BPA, BPF, BPS or DMSO control at different time points were generated by RNA-seq, using a BGISEQ-500 platform. Reads were filtered and aligned to the reference genome with Bowtie2. Results: BPA, BPF and BPS disrupted many processes, during mESC global and neural differentiations, in very similar manners. In fact, analogous gene categories were differentially regulated by the three chemicals, at each time point, particularly the ones involved in cell-matrix and cell-cell adhesions, signal transduction pathways, and medical conditions such as cardiovascular diseases and cancer.

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Effects of BPA, BPAF, and BPS on Endochondral Ossification and on the Transcriptome of Ex Vivo Murine Limb Bud Cultures

Project description:This SuperSeries is composed of the SubSeries listed below.

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