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Disentangling glial diversity in peripheral nerves at single-nuclei resolution [single-cell and single-nuclei RNA-seq]

ABSTRACT: Disentangling glial diversity in peripheral nerves at single-nuclei resolution [single-cell and single-nuclei RNA-seq]

PROVIDER: PRJNA754445 | ENA |

REPOSITORIES: ENA

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Disentangling glial diversity in peripheral nerves at single-nuclei resolution

Project description:Disentangling glial diversity in peripheral nerves at single-nuclei resolution

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Disentangling glial diversity in peripheral nerves at single-nuclei resolution [bulk and RiboTag RNA-seq]

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Disentangling glial diversity in peripheral nerves at single-nuclei resolution [single-cell and single-nuclei RNA-seq]

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Disentangling glial diversity in peripheral nerves at single-nuclei resolution [bulk and RiboTag RNA-seq]

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Project description:The peripheral nerve contains diverse cell types that support its proper function and maintenance. In this study, we analyzed multiple peripheral nerves using single-nuclei RNA sequencing, which allowed us to circumvent difficulties encountered in analyzing cells with complex morphologies via conventional single-cell methods. The resultant mouse peripheral nerve cell atlas highlights a diversity of cell types, including multiple subtypes of Schwann cells (SCs), immune cells and stromal cells. We identified a distinct myelinating SC subtype that expresses Cldn14, Adamtsl1 and Pmp2 and preferentially ensheathes motor axons. The number of these motor-associated Pmp2+ SCs is reduced in both an amyotrophic lateral sclerosis (ALS) SOD1G93A mouse model and human ALS nerve samples. Our findings reveal the diversity of SCs and other cell types in peripheral nerve and serve as a reference for future studies of nerve biology and disease.

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Disentangling glial diversity in peripheral nerves at single-nuclei resolution

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-12-06 | GSE182099 | GEO

Ambient RNA analysis of single-nuclei RNA-Seq data from the frontal cortex of C57BL/6J mice

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