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Gene epression profile of s-spheroids and t-spheroids differentiated from iPS cells

ABSTRACT: Gene epression profile of s-spheroids and t-spheroids differentiated from iPS cells

PROVIDER: PRJNA755339 | ENA |

REPOSITORIES: ENA

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Gene epression profile of s-spheroids and t-spheroids differentiated from iPS cells

Project description:For definitive endoderm differentiation, iPS cells (day0) were treated with 100 ng ml-1 activin A and 3μM CHIR99021 for 1 day and 100 ng ml-1 activin A for the following two days. Definitive endoderm was subsequently treated with 3uM CHIR99021 and 500ng ml-1 FGF4 for mid/hindgut differentiation. Mid/hindgut floating spheroids (fl; t-Spheroids) were collected from culture medium from day6 to day 8. On day6, mid/hindgut cells were dissociated into single cells and seeded onto EZSPHERE plate to generate suspension spheroids(EZ; s-Spheroids). RNA of fl spheroids and EZ spheroids were extracted using QIAGEN RNeasy kit.

2022-11-15 | GSE182230 | GEO

Gene profile in ovarian cancer cell spheroids

Project description:Gene profile in ovarian cancer cell spheroids

| PRJNA564046 | ENA

Gene expression profile of human melanocytes derived from iPS cells

Project description:This experiment was designed to show the similarity among normal human epidermal melanocytes, melanocytes derived from human 3F-induced pluripotent stem (iPS) cells, and human 3F-iPS cells. Human 3F-iPS cells without c-myc were established and then differentiated into melanocytes. The differentiated cells were collected and the gene expression profile was compared to normal human epidermal melanocytes (NHEMs) and orginal 3F-iPS cells.

2010-08-26 | E-GEOD-23805 | biostudies-arrayexpress

Dynamic changes in gene expression profile during differentiation of iPS into MSCs

Project description:Analysis of gene expression profile at different developmental stages of MSCs. iPS cells from human fibroblasts are differentiated into MSCs undergoing primitive streak and mesoderm. Results provide important information of dynamic changes in gene expression profile during MSC development.

2019-11-16 | GSE140450 | GEO

Proteomics of hepatocyte like cells differentiated from iPS cells

Project description:Proteomics and phosphoproteomics in hepatocyte like cells differentiated from iPS cells with gene mutation of V30M or Y114C.

2024-11-22 | PXD047155 | JPOST Repository

RNA sequencing of enterocytes differentiated from human iPS cells

Project description:RNA sequencing of enterocytes differentiated from human iPS cells

| PRJNA941880 | ENA

Expression data from undifferentiated and iPS cells differentiated to a myogenic fate [MYOG]

Project description:Here, we report the generation of human induced Pluripotent Stem (iPS) cell reporter line in which a venus fluorescent protein have been introduced into the MYOGENIN (MYOG) locus. We use microarrays to compare the transcriptome of MYOG-venus+ cells after 3 weeks of myogenic differentiation to that of undifferentiated iPS Satellite cells (SC) are muscle stem cells which can regenerate adult muscles upon injury. Most SC originate from PAX7-positive myogenic precursors set aside during development. While myogenesis has been studied in mouse and chicken embryos, little is known about human muscle development. Here, we use microarrays to compare the transcriptome of myogenic cells differentiated in vitro from human and mouse ES and iPS cells reporter cell lines. We generated fluorescent reporter lines in which a fluorescent protein was introduced into the loci coding for Pax7 (mouse ES and human iPS) or MYOG (human iPS). Mouse ES and human iPS cells were differentiated to the myogenic lineage in vitro for three weeks according to the protocols described in Chal et al, Nature Biotech 2015 and to Chal, Al Tanoury et al, Nature Protocols, 2016. Fluorescent Pax7 or MYOG-positive cells were FACS-sorted after 3-weeks of differentiation in vitro and we used Affymetrix microarrays to analyze the transcriptome of the purified mouse and human cell populations and compare it to the transcriptome of undifferentiated mouse ES or human iPS cells.

2020-04-21 | GSE148994 | GEO

Expression data from undifferentiated and iPS cells differentiated to a myogenic fate [hPAX7]

Project description:Here, we report the generation of human induced Pluripotent Stem (iPS) cell reporter line in which a venus fluorescent protein have been introduced into the PAX7 locus. We use microarrays to compare the transcriptome of PAX7-venus+ cells after 3 weeks of myogenic differentiation to that of undifferentiated iPS Satellite cells (SC) are muscle stem cells which can regenerate adult muscles upon injury. Most SC originate from PAX7-positive myogenic precursors set aside during development. While myogenesis has been studied in mouse and chicken embryos, little is known about human muscle development. Here, we use microarrays to compare the transcriptome of myogenic cells differentiated in vitro from human and mouse ES and iPS cells reporter cell lines. We generated fluorescent reporter lines in which a fluorescent protein was introduced into the loci coding for Pax7 (mouse ES and human iPS) or MYOG (human iPS). Mouse ES and human iPS cells were differentiated to the myogenic lineage in vitro for three weeks according to the protocols described in Chal et al, Nature Biotech 2015 and to Chal, Al Tanoury et al, Nature Protocols, 2016. Fluorescent Pax7 or MYOG-positive cells were FACS-sorted after 3-weeks of differentiation in vitro and we used Affymetrix microarrays to analyze the transcriptome of the purified mouse and human cell populations and compare it to the transcriptome of undifferentiated mouse ES or human iPS cells.

2020-04-22 | GSE149055 | GEO

Genome-wide nucleosome positioning maps from undifferentiated human iPS cells (hIPS) and iPS cells differentiated to neural progenitor cells (NPC).

Project description:We report the application of MNase-seq (Kent, Adams et al. 2011) to construct genome-wide nucleosome maps from human cells. To date, genome-wide changes in chromatin structure that occur during development in human cells have not been investigated widely. We have constructed and compared genome-wide chromatin maps from undifferentiated human iPS cells and and iPS cells differentiated to neural progenitor cells (NPC).

2019-04-24 | GSE117870 | GEO

RNA sequencing of enterocytes differentiated from human iPS cells [RNAseq1]

Project description:RNA sequencing of enterocytes differentiated from human iPS cells [RNAseq1]

| PRJNA941070 | ENA