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Transcriptomic analysis of the follicle associated epithelium of Peyer's Patches, intestinal villous epithelium and ileum from young and aged mice

ABSTRACT: Transcriptomic analysis of the follicle associated epithelium of Peyer's Patches, intestinal villous epithelium and ileum from young and aged mice

PROVIDER: PRJNA755594 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15500500_1.fastq.gz	Fastqsanger.gz
	SRR15500500_2.fastq.gz	Fastqsanger.gz
	SRR15500501_1.fastq.gz	Fastqsanger.gz
	SRR15500501_2.fastq.gz	Fastqsanger.gz
	SRR15500502_1.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of the follicle associated epithelium of Peyer's Patches, intestinal villous epithelium and ileum from young and aged mice

Project description:We have previously shown that aging affects the functional maturation of M cells in the follcile associated epithelium (FAE) of Peyer's patches in the small intestine. To further characterise how aging affects the FAE, mRNA-seq was performed on micro-dissected FAE and villous epithelial sheets as well as whole ileum from young (4 month old) and aged (26-28 month old) mice

2021-11-19 | GSE182252 | GEO

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Single-cell and spatial transcriptomics profile of porcine jejunum and ileum containing Peyer's patches

Project description:To establish better understanding of cells found in jejunal and ileal Peyer's patches of pigs, we utilized single-cell RNA sequencing scRNA-seq and spatial transcriptomics to recover and analyze cells and spatial regions from sections of jejunum and ileum containing Peyer's patches. Cells identified via single-cell RNA sequencing included B, T/innate lymphoid cell, myeloid, epithelial, and stromal lineage cells. Spatial dots recovered via spatial transcriptomics belonged to regions including villi, crypts, interfollicular/parafollicular zones, follicles, and muscularis. Overall, results provide new information on regional localization and transcriptional profiles of cells in the pig small intestine.

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Transcription profiling of mouse Peyers patch M cells, villous M-like cells, and intestinal epithelial cells

Project description:Separate populations of M cells have been detected in the follicle-associated epithelium of Peyerâs patches (PPs) and the villous epithelium of the small intestine, but the traits shared by or distinguishing the two populations have not been characterized. Our separate study has demonstrated that M cells are rare but M-like cells positive for lectin Ulex europaeus agglutinin-1 and our newly developed M cell-specific mAb NKM16-2-4 are induced by oral administration of cholera toxin in the duodenal villous epithelium. Here, we determined the gene expression of PP M cells, villous M-like cells, and intestinal epithelial cells (IECs) isolated by a novel approach using FACS. Specific expression of glycoprotein 2 (GP2) and MARCKS-like protein (MLP) by PP M cells and not villous M-like cells was confirmed by additional mRNA and protein analyses. Comprehensive gene profiling also suggested that villous M-like cells share traits of both PP M cells and IECs, a finding that is supported by their unique expression of specific chemokines. The genome-wide assessment of gene expression facilitates discovery of M cell-specific molecules and enhances the molecular understanding of M cell immunobiology. Experiment Overall Design: Epithelial cells in duodenal Peyer's patches or villi of BALB/c mice treated with or without cholera toxin were dissociated and stained with NKM16-2-4 monoclonal antibody (mAb)-FITC, lectin UEA-1-PE, and anti-CD45 mAb-APC-Cy7. Naive PP M cells (NKM16-2-4+, UEA-1+, and CD45- cells), CT-induced villous M-like cells (NKM16-2-4+, UEA-1+, and CD45- cells), and naive intestinal epithelial cells (NKM16-2-4-, UEA-1-, and CD45- cells) were isolated by FACS. Affymetrix GeneChip Mouse Genome 430 2.0 Array was used in triplicate for each cell fraction.

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Project description:The Toxoplasma type I ROP16 kinase directly activates the host STAT3 and STAT6 transcription factors and when transgenically expressed in the orally virulent type II strain, promotes host resistance to oral challenge. The transcriptional profile of type II and II+ROP16I infected Peyer's patches and intestines from orally infected mice on day 5 was determined to elucidate host signaling pathways and molecular gene targets that correlate with protective immunity in the gut of orally challenged animals C57BL/6 female mice were orally gavaged with 1000 tissue cysts of the type II or II+ROP16I strain. On Day 5 of infection the Peyer's patches and intestines were analyzed for parasite burden by bioluminescence imaging. Individual Peyer's patches and intestinal sections, corresponding to the middle third of the intestine (~jejunum), that had equivalent parasite burden were snap frozen in liquid nitrogen. The samples were crushed using a sterile 16 G needle in tubes on dry ice. In the case of the intestine, the sample was resuspended in TRizol, extruded through a needle using a syringe and RNA was isolated according to manufacturer's protocol; for the Peyer's patch, the sample was suspended in cell lysis buffer and RNA isolated using the RNeasey kit (Invitrogen) according to the manufacter's protocol.

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