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RNA-seq of ITPR3 knockout in SW480 colorectal cancer metastases

ABSTRACT: RNA-seq of ITPR3 knockout in SW480 colorectal cancer metastases

PROVIDER: PRJNA755598 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15500556.fastq.gz	Fastqsanger.gz
	SRR15500557.fastq.gz	Fastqsanger.gz
	SRR15500558.fastq.gz	Fastqsanger.gz
	SRR15500559.fastq.gz	Fastqsanger.gz
	SRR15500560.fastq.gz	Fastqsanger.gz

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RNA-seq of ITPR3 knockout in SW480 colorectal cancer metastases

Project description:Here we analyze the transcriptional profiles of liver metastases formed by sgCtrl versus sgITPR3 SW480 colorectal cancer cells. We find downregulated genes in sgITPR3 SW480 metastases to be enriched for pathways related to immune response and non-canonical NFkB signaling.

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RNA-seq of ITPR3 and RELB knockout in SW480 under normoxia and hypoxia

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Expression analysis of colorectal cancer cell line SW480 expressing FOXC2

Project description:Investigation of whole genome gene expression level changes in a colorectal cancer cell line SW480 expressing FOXC2, compared to the pBabe control cells. Genes associated with metastasis regulated by FOXC2 in colorectal cancer were analysed. The role of FOXC2 in breast cancer metastasis are further described in Mani SA, Yang J et al. Mesenchyme Forkhead 1 (FOXC2) plays a key role in metastasis and is associated with aggressive basal-like breast cancers. PNAS 2007; 104: 10069-10074 . A six chip study using total RNA recovered from three separate cultures of SW480/pBabe and three separate cultures of SW480/FOXC2. Each chip measures the expression level of 45033 genes from SW480/pBabe or SW480/FOXC2.

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MicroRNA expression profile between human colorectal cancer SW480 and SW620 cell line

Project description:Two isogenic human colorectal cancer cell lines (primary SW480 cell line and its lymph node metastatic variant SW620 cell line),as an in vitro metastatic model. We have demonstrated that SW620 cell line possesses high metastasis potential and SW480 cell linepossesses low metastatic potential. We want to compare the whole cell microRNAs profiles of two isogenic colorectal cancer cell lines (SW480 and SW620 cell line), to gain an insight into the molecular events of colon cancer metastasis.

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RNA-seq of ITPR3 and RELB knockout in SW480 under normoxia and hypoxia

Project description:RNA-seq of ITPR3 and RELB knockout in SW480 under normoxia and hypoxia

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Exosomal natural antisense transcripts released from colorectal cancer SW480 cells into culture supernatants

Project description:In the present study, natural antisense transcripts (NATs), transcribed from reverse strand deoxynucleic acids (DNAs) of genes, into exosomes released from colorectal cancer SW480 cells were searched using an sense/antisense-custom microarray. SW480 cells were cultured with serum-free RPMI1640 for 48 h. Then, culture media were collected, centrifugated, and filtrated using 0.22-um filters. Exosomes from culture media were collected by ultracentrifugation.

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Expression analysis of colorectal cancer cell line SW480 expressing FOXC2

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Exosomal natural antisense transcripts released from colorectal cancer SW480 cells into culture supernatants

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