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M6A-seq of Min6 cells were transfected with the GR cDNA expression vector or control vector

ABSTRACT: m6A-seq of Min6 cells were transfected with the GR cDNA expression vector or control vector

PROVIDER: PRJNA755630 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15501900_1.fastq.gz	Fastqsanger.gz
	SRR15501900_2.fastq.gz	Fastqsanger.gz
	SRR15501901_1.fastq.gz	Fastqsanger.gz
	SRR15501901_2.fastq.gz	Fastqsanger.gz
	SRR15501902_1.fastq.gz	Fastqsanger.gz

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RNA-seq of Min6 cells were transfected with the GR cDNA expression vector or control vector

Project description:In order to investigated the underlying molecular mechanism of GR’s effects, we transfected the Min6 cell with the GR cDNA expression vector or control vector, and then extracted RNA of Min6 cells for RNA sequencing. We studied the gene-expression differences resulting in GR overexpress. A total of 21542 gene transcripts was captured,and 233 genes showed significant expression differences (p-value<0.05,|log2Foldchange|>1) between the two group. Further GO analysis and KEGG analysis found that GR overactivation instigates dysregulated autophagy in beta-cells

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m6A-seq of Min6 cells were transfected with the GR cDNA expression vector or control vector

Project description:To further explore the underlying molecular mechanism of GR’s effects, we transfected the Min6 cell with the GR cDNA expression vector or control vector, and then extracted RNA of Min6 cells. M6A antibody was used to specifically recognize m6A on mRNA. methylation modified RNA fragments were obtained by immunoprecipitation and used for high-throughput sequencing. By comparing the captured RNA fragments, we found that there were 22381 difference peaks between GR overexpression group and control group, among which 3659 binding peaks were significantly different.Further analysis the MeRIP sequencing results of MIN6 cells overexpressing GR revealed that decreased m6A was detected in a large number of autophagy-related mRNAs.

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RNA-seq of Min6 cells were transfected with the GR cDNA expression vector or control vector

Project description:RNA-seq of Min6 cells were transfected with the GR cDNA expression vector or control vector

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Project description:RNA-seq and m6A-seq of Min6 cells were transfected with the GR cDNA expression vector or control vector

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RNA-seq and m6A-seq of Min6 cells were transfected with the GR cDNA expression vector or control vector

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