Project description:RNA sequencing (RNA-seq) experiment was performed on 18 samples of Nitzschia laevis cells collected at 3 h, 6 h and 12 h after inoculation in treated medium with glucose addition (+G) and control group (-G) respectively. The de novo assembly totally generated 16650 unigenes.
Project description:Precise regulation of cell proliferation and differentiation is a key factor for diversity in morphology during organogenesis. Rice palea comprises two distinct regions, the marginal region of palea (MRP) and the body of palea (BOP), which exhibit heterogeneity in cellular composition and behavior. This study reveals that REP1 promotes epidermal cell proliferation and differentiation in the BOP, forming hard silicified protrusion cells, by regulating the cyclin-dependent kinase gene, OsCDKB1;1. Conversely, OsMADS6 and OsMADS32 are expressed exclusively in the MRP, where they limit the division rates of epidermal cells by inhibiting OsCDKB2;1 gene expression, promoting endoreduplication and resulting in the formation of elongated epidermal cells. Furthermore, the OsMADS6-OsMADS32 complex and REP1 exhibit reciprocal inhibition to fine-tune the balance between cell division and differentiation during palea morphogenesis. Therefore, our study highlights the importance of controlling cellular heterogeneity in shaping plant morphological diversity.
Project description:The lemma, the palea and the awn of a barley spike are photosynthetic organs and supply the developing seed with carbohydrates. In addition, the lemma and the palea cover the seed and protect it from pathogens and insects. In this study, we compared gene expression among the lemma, the palea, the awn and the developing seed of barley at the grain-filling stage (Zadok scale 83) using the Barley1 Genome Array in order to identify genes that determine the primary function of these organs. Experiment Overall Design: Lemma, palea, awn and seed were collected at grain-filling stage (Zadok scale 83) to compare differences in gene expression. For each organ three biological replications were collected on three different dates. On each of the three dates, one replication of each organ was collected from plants grown in separate pots using a randomized complete block design (RCBD). In ths design, organ type was the treatment effect and date of sample collection (replication) was the block. To get enough tissue for RNA extraction, each replication for each organ was collected from four plants in the same pot. RNA from each replicate sample was hybridized to individual Barley1 GeneChips. Thus, for four organs with three replications (blocks), a total of 12 Barley1 GeneChips were used.