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SFB-driven regulation in small intestinal epithelial cells during infection

ABSTRACT: SFB-driven regulation in small intestinal epithelial cells during infection

PROVIDER: PRJNA757128 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15571156_1.fastq.gz	Fastqsanger.gz
	SRR15571156_2.fastq.gz	Fastqsanger.gz
	SRR15571157_1.fastq.gz	Fastqsanger.gz
	SRR15571157_2.fastq.gz	Fastqsanger.gz
	SRR15571158_1.fastq.gz	Fastqsanger.gz

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SFB-driven regulation in small intestinal epithelial cells during infection

Project description:Expression in intestinal epithelial cells from intestine of germ-free and segmented filamentous bacteria (SFB)-monoassociated mice during infection. Intestinal epithelial cells were harvested from the terminal ileum of germ-free or SFB mice infected with Citrobacter rodentium for 6 days. RNA was isolated using RNeasy Kit (Qiagen). Sequencing was performed using the Illumina HiSeq2500. Reads were mapped to the mm10 genome using Bowtie.

2022-11-08 | GSE182630 | GEO

SFB-driven epigenetic regulation in small intestinal epithelial cells

Project description:H3K27Ac enrichment in intestinal epithelial cells from intestine of germ-free and segmented filamentous bacteria (SFB)-monoassociated mice. Intestinal epithelial cells were harvested from the terminal ileum of germ-free or SFB mice. Chromatin immunoprecipitation was performed with anti-H3K27Ac. Sequencing was performed using the Illumina HiSeq2500. Reads were mapped to the mm10 genome using Bowtie.

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SFB-driven epigenetic regulation in small intestinal epithelial cells

Project description:SFB-driven epigenetic regulation in small intestinal epithelial cells

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Microbiota-driven regulation in small intestinal epithelial cells [RNA-seq]

Project description:Microbiota-driven regulation in small intestinal epithelial cells [RNA-seq]

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RNA-seq data of intestinal epithelial cells

Project description:We report that adhesion of microbes to intestinal epithelial cells is a critical cue for Th17 induction. SFB colonized in the intestine of mice can adhere to mouse small intestinal epithelial cells and induce intestinal Th17 cells. However, SFB colonized in rats cannot adhere to mouse intestinal epithelial cells and induce Th17 cells. Likewise, Citrobacter rodentium (WT) can adhere to mouse colonic epithelial cells and induce Th17 cells, but non-adherent mutant of C. rodentium (Δeae) cannot induce Th17 cells. To examine the influence of adherent bacteria on intestinal epithelial cells, we performed RNA seq. Germ free mice were orally inoculated with M-SFB or R-SFB and total RNA was isolated from small intestinal epithelial cells 1 week after inoculation. Alternatively, germ free mice were orally inoculated with C. rodentium WT or eae mutant and total RNA was isolated from colonic epithelial cells 5 days after inoculation. The gene expression of small intestinal epithelial cells isolated from small intestine of germ free mice (2 mice), mice monocolonized with M-SFB (2 mice) or R-SFB (3 mice), and colon of germ free mice (3 mice), mice monocolonized C. rodentium WT (3 mice) or eae mutant (3 mice).

2015-09-24 | E-GEOD-71734 | biostudies-arrayexpress

RNA-seq data of intestinal epithelial cells

2015-09-24 | GSE71734 | GEO

Single cell profiling of SFB induced transcriptomic changes in small intestinal epithelium and immune system.

Project description:In this project we profiled small intestinal epithelium, lamina propria immune cells as well as intraepithelial immune cells from 5-weeks old WT mice derived from Jackson laboratories and littermate colonized with segmented filamentous bacteria (SFB) 2 weeks prior to analysis, using 10x droplet-based single-cell RNA sequencing.

2023-06-01 | E-MTAB-12391 | biostudies-arrayexpress

Microbiota-driven regulation in small intestinal epithelial cells [RNA-seq]

Project description:We compare transcriptomic profiles of intestinal epithelial cells obtained from the small intestine of germ-free and conventionally-caged mice. Intestinal epithelial cells were harvested from the intestine of conventional or germ-free C57Bl6J mice. Directional polyA RNA-seq was performed on RNA fom cells using standard Illumina protocols. Microbiota induce decreased expression of the Clec2e gene.

2019-05-26 | GSE128362 | GEO

Microbiota-driven regulation and epigenetic alterations in intestinal epithelial cells

Project description:Microbiota-driven regulation and epigenetic alterations in intestinal epithelial cells

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Gene expressions specifically induced by the presence of SFB

Project description:To investigate SFB-host interactions and the host response to SFB, we compared gene expression profiles in terminal ileal mucosa collected from 5 SFB-positive and 5-negative age-matched patients identified from SFB PCR. We observed that 472 genes were different between the two groups (P < 0.05). Among them, 290 genes were up-regulated and 182 were down-regulated. GO biological pathway analysis of up-regulated genes revealed positive regulation of T-cell differentiation and activation pathways were enhanced (P < 0.001), suggesting that SFB colonization stimulated the human immune system, specifically T-cell maturation. Indeed, enhanced expression of Cd3e, Ifng, IL10, Foxp3 was observed in terminal ileal mucosa of 5 SFB-positive patients.

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