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ABSTRACT: Synechocystis 6803 suppressor mutants

PROVIDER: PRJNA757148 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15573085_1.fastq.gz	Fastqsanger.gz
	SRR15573085_2.fastq.gz	Fastqsanger.gz
	SRR15573086_1.fastq.gz	Fastqsanger.gz
	SRR15573086_2.fastq.gz	Fastqsanger.gz
	SRR15573087_1.fastq.gz	Fastqsanger.gz

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Synechocystis sp. PCC 6803

Project description:Synechocystis 6803 suppressor mutants

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Examination of gene expression levels in mutants of phytochrome-related protain

Project description:To identify novel phototransduction pathways in cyanobacteria, mutants defective for phytochrome-related proteins in Synechocystis sp. PCC 6803 that exhibit increased or decreased gene expression levels. were screened. Keywords: array-based

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Label-free quantitative proteomic analysis of DNA methyltransferase mutants of the cyanobacterium Synechocystis sp. PCC 6803 – Analysis of total protein extracts

Project description:Total protein and membrane-enriched fractions of Synechocystis sp. PCC 6803 wild type (WT) and mutant strains affected with inactivated DNA methyltransferase genes were analyzed by LC-HDMSE. The mutation of the DNA methyltransferase gene sll0729 encoding M.Ssp6803II initially led to a strong phenotype, including a lowered chlorophyll/phycocyanin ratio, impaired growth, and alterations in gene expression. Prolonged cultivation revealed instability of the initially obtained phenotype. Colonies showing normal pigmentation and WT-like growth appeared regularly and in high frequencies on agar plates. These colonies represent suppressor mutants, since the sll0729 gene is still completely inactivated and methylation of the M.Ssp6803II target GGCC sites does not occur. The proteomic analysis comprises the WT, two strains of suppressor mutants sll0729_1 and sll0729_15 as well as mutants of the DNA methyltransferase genes slr6095 and sll8009. For each of these five strains we investigated three biological replicates. Comparisons between the WT and these two suppressor mutant strains, but also between them and the slr6095 and sll8009 mutants enabled the detection of expression differences, which are specifically linked to the absence of M.Ssp6803II-related DNA methylation.

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Label-free quantitative proteomic analysis of DNA methyltransferase mutants of the cyanobacterium Synechocystis sp. PCC 6803 – Analysis of the membrane-enriched fraction

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How cyanobacteria pose new problems to old methods: Challenges in microarray time series analysis

Project description:Quantification of circadian gene expression in WT Synechocystis sp. PCC 6803 cells

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Evidence for a major role of antisense RNAs in cyanobacterial gene regulation

Project description:The model cyanobacterium Synechocystis sp. PCC 6803 was used for a systematic survey on the number and types of antisense (as)RNAs. A tiled microarray was constructed with probes for both strands of the genes or intergenic spacers with candidate transcripts predicted by an algorithm and an equally sized and distributed control set. The resulting 102,739 individual probes cover an accumulated length of 1,441,146 nt or about 40% of the total chromosome sequence of Synechocystis 6803. Hybridization of this array with total RNA isolated from cultures raised under different growth conditions identified a high number of transcripts from intergenic spacers and in antisense orientation to known genes (natural cis-asRNAs). Extrapolated to the whole genome, around 10% of all open reading frames in Synechocystis 6803 may have a corresponding asRNA, suggesting a much more important role of chromosomally encoded asRNAs in bacteria then anticipated so far. Keywords: stress response

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Evidence for a major role of antisense RNAs in cyanobacterial gene regulation

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Synechocystis sp. PCC 6803 trophic condition

Project description:Targeted proteome analysis of Synechocystis sp. PCC 6803 under photoautotrophic and mixotrophic conditions

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Active site of cyanobacterial RNA polymerase compensates for the absence of proofreading factors [Synechoscystis sp]

Project description:We found that cyanobacterial RNA polymerase possesses very efficient intrinsic proofreading ability. This ability allows model species of cyanobacteria, Synechocystis sp PCC 6803 to keep in vivo level of transcriptional mistakes close to that of E.coli.

2018-08-15 | GSE115134 | GEO

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