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Gene expression and intron retention analysis of 15.5K-knockdown human cells

ABSTRACT: Gene expression and intron retention analysis of 15.5K-knockdown human cells

PROVIDER: PRJNA757717 | ENA |

REPOSITORIES: ENA

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Gene expression and intron retention analysis of 15.5K-knockdown human cells

Project description:15.5K is a RNA binding protein involved in the Minor Spliceosome. Here, we used shRNAs to knock down 15.5K in HEK293T cells to analysis the function of 15.5K in gene expression and intron retention.

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Gene expression and intron retention analysis of ZCRB1-knockdown human cells

Project description:Gene expression and intron retention analysis of ZCRB1-knockdown human cells

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Project description:We present an analysis of intron retention under stress from two different drugs and their combinations in yeast Saccharomyces cerevisiae. We previously established isogrowth profiling, a method to abstract the non-specific effects of growth rate inhibition from the specific effect of perturbation by a small molecule: two drugs are used at varied ratios, but at fixed overall growth inhibition. Here, cycloheximide and LiCl were used at seven different ratios along the 50% growth inhibition isobole and the total ribodepleted RNA was sequenced. This allowed us to gauge the changes in intron retention due to the used drugs, while ensuring that the effects are not caused by growth inhibition. We found a prominent increase in intron retention under LiCl treatment that preferentially affects introns contained in the transcripts of ribosomal proteins.

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Gene expression and intron retention analysis of bktRNA1-depleted human cells

Project description:Backwark kink-turn RNA 1 (bktRNA1) is a novel post-transcriptional regulator with a role in RNA modification. Here, we show that human bktRNA1 interacts with 15.5K and FBL and guides 2’-O-methylation in the 8th of U12 snRNA. Given that U12 plays crucial role in minor intron splicin, we knockouted bktRNA1 in HCT116 cells and examined the effects of the modification on minor splicing by transcriptome analyses. Our work defines a novel functional interaction bwtween bktRNA1 and RNA processing and highlighs that a single modification alteration may contribute to global impairment of the splicing machinery.

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Project description:Gene expression and intron retention analysis of bktRNA1-depleted human cells

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Project description:We explored intron retention patterns in normal breast epithelial cells (MCF10A) and estrogen receptor positive (ER+) breast cancer cells (MCF7).

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Targeted intron retention and excision for rapid gene regulation in response to neuronal activity

Project description:A substantial population of intron retentions are stably maintained in poly-adenylated transcripts and a subset of them are excised upon neuronal stimulation. These regulated intron retention events in fully transcribed RNAs represent a mechanism to rapidly mobilize a pool of mRNAs in response to neuronal activity.

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