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Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [scRNA-seq]

ABSTRACT: Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [scRNA-seq]

PROVIDER: PRJNA757786 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15614139_1.fastq.gz	Fastqsanger.gz
	SRR15614139_2.fastq.gz	Fastqsanger.gz
	SRR15614140_1.fastq.gz	Fastqsanger.gz
	SRR15614140_2.fastq.gz	Fastqsanger.gz
	SRR15614141_1.fastq.gz	Fastqsanger.gz

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Derivation of functional trophoblast stem cells from human primed pluripotent stem cells

Project description:Derivation of functional trophoblast stem cells from human primed pluripotent stem cells

| PRJNA757782 | ENA

Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [bulk RNA-seq]

Project description:Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [bulk RNA-seq]

| PRJNA757785 | ENA

Derivation of trophoblast stem cells from naïve human pluripotent stem cells [ATAC-seq]

Project description:Naïve human pluripotent stem cells (hPSCs) provide a unique experimental platform of cell fate decisions during pre-implantation development, but their lineage potential remains incompletely characterized. As naïve hPSCs share transcriptional and epigenomic signatures with trophoblast cells, it has been proposed that the naïve state may have enhanced predisposition for differentiation along this extraembryonic lineage. Here we examined the trophoblast potential of isogenic naïve and primed hPSCs. We found that naïve hPSCs can directly give rise to human trophoblast stem cells (hTSCs) and undergo further differentiation into both extravillous and syncytiotrophoblast. In contrast, primed hPSCs do not support hTSC derivation, but give rise to non-self-renewing cytotrophoblasts in response to BMP4. Global transcriptome and chromatin accessibility analyses indicate that hTSCs derived from naïve hPSCs acquire features of pre-implantation trophectoderm. The derivation of hTSCs from naïve hPSCs will enable elucidation of early mechanisms that govern normal human trophoblast development and associated pathologies.

2020-02-13 | GSE138761 | GEO

Derivation of trophoblast stem cells from naïve human pluripotent stem cells [RNA-seq]

2020-02-13 | GSE138688 | GEO

Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [scRNA-seq]

Project description:Trophoblast stem cells (TSC) have recently been derived from human embryos, and early first trimester placenta; however, aside from ethical challenges, the unknown disease potential of these cells limits their scientific utility. We have previously established a BMP4-based two-step protocol for differentiation of primed human pluripotent stem cells (hPSC) into functional trophoblast; however, those trophoblast could not be maintained in a self-renewing TSC-like state. Here, we use the first step from this protocol, followed by a switch to a newly-developed TSC media, to derive bona-fide TSC. We show that these cells resemble placenta- and naïve hPSC-derived TSC, based on their transcriptome, as well as in vitro and in vivo differentiation potential. We conclude that primed hPSC can be used to generate functional TSC, through a simple protocol which can be applied to a widely-available set of existing hPSC, including induced pluripotent stem cells derived from patients with known birth outcomes.

2022-05-16 | GSE182790 | GEO

Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [bulk RNA-seq]

2022-05-16 | GSE182789 | GEO

Derivation of trophoblast stem cells from naïve human pluripotent stem cells

Project description:Derivation of trophoblast stem cells from naïve human pluripotent stem cells

| PRJNA577070 | ENA

Derivation of trophoblast stem cells from naïve human pluripotent stem cells [ATAC-seq]

Project description:Derivation of trophoblast stem cells from naïve human pluripotent stem cells [ATAC-seq]

| PRJNA577096 | ENA

Derivation of trophoblast stem cells from naïve human pluripotent stem cells [RNA-seq]

Project description:Derivation of trophoblast stem cells from naïve human pluripotent stem cells [RNA-seq]

| PRJNA576801 | ENA

Human primed and naive PSCs are equally potent in differentiating into bona fide trophoblast stem cells (RNA-Seq)

Project description:Cells of the trophoblast lineage constitute the major part of placental tissues in higher mammals. Recent derivation of human trophoblast stem cells (TSC) from placental cytotrophoblasts (CT) and from blastocyst opens new opportunities for studying development and function of human placenta. Here we report that inhibition of TGF pathway leads to direct and robust conversion of primed human pluripotent stem cells into TSC. The resulting cell lines exhibit self-renewal, are able to differentiate into the main trophoblast lineages, and present RNA and epigenetic profiles that are indistinguishable from the TSC lines derived from placenta or blastocyst. Furthermore, activation of YAP pathway is necessary and sufficient for this conversion.

2022-09-21 | GSE192917 | GEO

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