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Transcriptomic profiling of adult-onset asthma related to damp and moldy buildings and idiopathic environmental intolerance

ABSTRACT: Transcriptomic profiling of adult-onset asthma related to damp and moldy buildings and idiopathic environmental intolerance

PROVIDER: PRJNA757930 | ENA |

REPOSITORIES: ENA

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Transcriptomic profiling of adult-onset asthma related to damp and moldy buildings and idiopathic environmental intolerance [nasal biopsy]

Project description:The objective of the study was to characterize distinct endotypes of asthma related to damp and moldy buildings and to evaluate the potential molecular similarities with idiopathic environmental intolerance (IEI). The nasal biopsy transcriptome of 88 study subjects was profiled using samples obtained at baseline.

2021-10-20 | GSE182797 | GEO

Transcriptomic profiling of adult-onset asthma related to damp and moldy buildings and idiopathic environmental intolerance [blood]

Project description:The objective of the study was to characterize distinct endotypes of asthma related to damp and moldy buildings and to evaluate the potential molecular similarities with idiopathic environmental intolerance (IEI). The blood cell transcriptome of 88 study subjects was profiled using peripheral blood mononuclear cell (PBMC) samples obtained at baseline.

2021-10-20 | GSE182503 | GEO

Transcriptomic profiling of adult-onset asthma related to damp and moldy buildings and idiopathic environmental intolerance [blood]

Project description:Transcriptomic profiling of adult-onset asthma related to damp and moldy buildings and idiopathic environmental intolerance [blood]

| PRJNA756532 | ENA

Transcriptomic profiling of adult-onset asthma related to damp and moldy buildings and idiopathic environmental intolerance [nasal biopsy]

Project description:Transcriptomic profiling of adult-onset asthma related to damp and moldy buildings and idiopathic environmental intolerance [nasal biopsy]

| PRJNA758097 | ENA

Transcriptomic Profiling of Adult-Onset Asthma Related to Damp and Moldy Buildings and Idiopathic Environmental Intolerance.

Project description:A subset of adult-onset asthma patients attribute their symptoms to damp and moldy buildings. Symptoms of idiopathic environmental intolerance (IEI) may resemble asthma and these two entities overlap. We aimed to evaluate if a distinct clinical subtype of asthma related to damp and moldy buildings can be identified, to unravel its corresponding pathomechanistic gene signatures, and to investigate potential molecular similarities with IEI. Fifty female adult-onset asthma patients were categorized based on exposure to building dampness and molds during disease initiation. IEI patients (n = 17) and healthy subjects (n = 21) were also included yielding 88 study subjects. IEI was scored with the Quick Environmental Exposure and Sensitivity Inventory (QEESI) questionnaire. Inflammation was evaluated by blood cell type profiling and cytokine measurements. Disease mechanisms were investigated via gene set variation analysis of RNA from nasal biopsies and peripheral blood mononuclear cells. Nasal biopsy gene expression and plasma cytokine profiles suggested airway and systemic inflammation in asthma without exposure to dampness (AND). Similar evidence of inflammation was absent in patients with dampness-and-mold-related asthma (AAD). Gene expression signatures revealed a greater degree of similarity between IEI and dampness-related asthma than between IEI patients and asthma not associated to dampness and mold. Blood cell transcriptome of IEI subjects showed strong suppression of immune cell activation, migration, and movement. QEESI scores correlated to blood cell gene expression of all study subjects. Transcriptomic analysis revealed clear pathomechanisms for AND but not AAD patients. Furthermore, we found a distinct molecular pathological profile in nasal and blood immune cells of IEI subjects, including several differentially expressed genes that were also identified in AAD samples, suggesting IEI-type mechanisms.

| S-EPMC8508786 | biostudies-literature

Transcriptomic profiling of adult-onset asthma related to damp and moldy buildings and idiopathic environmental intolerance

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-10-20 | GSE182798 | GEO

Expression analysis of DamP mRNAs using expressed molecular barcodes

Project description:We have generated 979 yeast strains in which the natural 3' UTR of essential gene mRNAs has been replaced by the same long 1.4 kb artificial 3' UTR (DAmP modification). Nonsense mediated mRNA decay (NMD) of these mRNA reporters was tested by using Agilent barcode microarrays by taking advantage of molecular barcodes introduced just downstream the stop codon during strain construction. We introduced in each DAmP strain either a neutral mutation (deletion of YEL068C) or the deletion of essential factors for NMD: NAM7 and NMD2. The resulting haploid cells were tested for changes in DAmP mRNA levels by comparing an RNA sample with a DNA genomic sample. Differences between the samples indicate the sensibility of the DAmP mRNAs to degradation through NMD. The large scale results were validated by Q-PCR analysis of individual strains. A strong negative correlation between the level of destabilization elicited by a long 3' UTR and the size of the coding sequence suggests that long ORF mRNAs can escape NMD even in the presence of a long 3' UTR. Three samples were analysed and for each sample a technical replicate was performed, starting from the raw cellular material. YEL068C samples represent the reference population while NMD2 and NAM7 samples are the ones important for the analysis. Only a very limited part of the Agilent barcode microarray signal (barcodes corresponding to the “up” tag in the DamP strain) was used for the interpretation of the data.

2014-01-10 | E-GEOD-53954 | biostudies-arrayexpress

Expression analysis of DamP mRNAs using expressed molecular barcodes

2014-01-10 | GSE53954 | GEO

Saccharomyces cerevisiae deletion and DAmP collections chemogenomic screen with Selenomethionine

Project description:Growth assay in the presence of Selenomethionine that uses the barcoded collections of yeast gene modification (deletion or DamP) to identify strains that are hypersensitive to the presence of the aminoacid.

2017-03-17 | GSE84340 | GEO

Mortars with Crushed Lava Granulate for Repair of Damp Historical Buildings.

Project description:In this paper, crushed lava granulate was used as full silica sand replacement in composition of repair mortars based on hydrated lime, natural hydraulic lime, or cement-lime binder. Lava granules were analyzed by X-ray fluorescence analysis (XRF), X-ray diffraction (XRD), and scanning electron microscopy (SEM). Particle size distribution of both silica and lava aggregates was assessed using standard sieve analysis. Hygrothermal function of the developed lightweight materials was characterized by the measurement of complete set of hygric, thermal, and structural parameters of the hardened mortar samples that were tested for both 28 days and 90 days cured specimens. As the repair mortars must also meet requirements on mechanical performance, their compressive strength, flexural strength, and dynamic Young's modulus were tested. The newly developed mortars composed of lava aggregate and hydrated lime or natural hydraulic lime met technical, functional, compatibility, and performance criteria on masonry and rendering materials, and were found well applicable for repair of historically valuable buildings.

| S-EPMC6862693 | biostudies-literature

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