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High-throughput sequencing Analysis of Transcriptomes of depolarized mouse BMDM cell

ABSTRACT: High-throughput sequencing Analysis of Transcriptomes of depolarized mouse BMDM cell

PROVIDER: PRJNA758996 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15673191_1.fastq.gz	Fastqsanger.gz
	SRR15673191_2.fastq.gz	Fastqsanger.gz
	SRR15673192_1.fastq.gz	Fastqsanger.gz
	SRR15673192_2.fastq.gz	Fastqsanger.gz
	SRR15673193_1.fastq.gz	Fastqsanger.gz

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High-throughput sequencing Analysis of Transcriptomes of depolarized mouse BMDM cell

Project description:Purpose:Mouse BMDM is the universal cell type of studying innate immunity.This study was to analyze LPS induced innate immune respone and the relationship between the membrane potential and LPS induced innate immune respone Methods: WT BMDM cells were treated with or without LPS for 6 hours in the pressence of membranepotential depolarization stimuli. Then mRNA profiles of these samles were generated by High-throughput sequencing analysis, in triplicate, using Illumina HiSeq /Novaseq or MGI2000. And the differencial mRNA profiles were analyzed. Results: mRNA profiles of more than 20000 genes were analyzed, and differencial expression profiles were compared . Conclusions: Our study represents the first detailed analysis of transcriptomes of WT and membrane potential depolarized macrophages, with biologic replicates, generated by RNA-seq technology. This data was useful to analyze the relationship between membrane potential and LPS induced innate immune respone

2022-04-05 | GSE183067 | GEO

High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes

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High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes

Project description:Purpose: Mouse BMDM is the universal cell type of studying innate immunity.This study was to analyze LPS induced innate immune respone and the relationship between serine synthesis pathway and LPS induced innate immune respone Methods: BMDM cells were treated with or without LPS for 6 hours in the pressence of phgdh inhibitor or not. Then mRNA profiles of these samles were generated by High-throughput sequencing analysis, in triplicate, using illumina HiSeqTM 2000. And the differencial mRNA profiles were analyzed. Results: mRNA profiles about 23002 genes were analyzed, and differencial expression profiles were compared . Conclusions: Our study represents the first detailed analysis of transcriptomes of mouse BMDM treated with LPS and phgdh inhibitor, with biologic replicates, generated by RNA-seq technology. This data was useful to analyze the relationship between serine synthesis pathway and LPS induced innate immune respone

2019-09-23 | GSE126084 | GEO

High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes after LPS Challenge

Project description:High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes after LPS Challenge

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High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes after tumor supernatants induction

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High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes after LPS Challenge

Project description:Purpose: Mouse BMDM is the universal cell type of studying innate immunity.This study was designed to analyze LPS induced innate immune respone and the gene expression in WT and HDAC3 KO BMDMs. Methods: BMDM cells from Hdac3f/f and Lyz2-Cre-Hdac3f/f were treated with or without LPS for 4 hours. Then mRNA profiles of these samles were generated by High-throughput sequencing analysis, in triplicate, using illumina HiSeqTM2000/MiSeq. And the differencial mRNA profiles were analyzed. Results: mRNA profiles were analyzed, and differencial expression profiles were compared . Conclusions: Our study demostrated the transcriptional profiles of BMDMs from Hdac3f/f and Lyz2-Cre-Hdac3f/f mice upon LPS challenge, with biologic replicates, generated by RNA-seq technology.

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High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes after tumor supernatants induction

Project description:The tumor microenvironment (TME) is a unique niche governed by constant crosstalk within and across all intratumoral cellular compartments. In particular, intratumoral high potassium has shown its immune-suppression potency on T cells. However, as a pan-cancer characteristic related to local necrosis, the impact of this ionic disturbance on innate immunity is still unknown. Here, using both murine and human samples, we reveal that intratumoral high potassium leads to profound suppression of the anti-tumor capacity of tumor-associated macrophages (TAMs). We report the high-throughput profiling of the transcriptomes of murine primary BMDMs after tumor conditioned medium (TCM) stimulation.

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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Cd226-/- BMDM Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare WT-BMDM vs. cd226-BMDM transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis

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High-throughput sequencing Transcriptomes Analysis of WT and Kcnj2 KO mouse BMDMs treated with LPS or kir2.1 inhibitor

Project description:Purpose:Mouse BMDM is the universal cell type of studying innate immunity.This study was to analyze LPS induced innate immune response and the relationship between the inward rectifier potassium channel Kir2.1 and LPS induced innate immune respone Methods: WT and Kcnj2 KO BMDM cells were treated with or without LPS for 6 hours in the presence of kir2.1 inhibitor ML133 or not. Then mRNA profiles of these samles were generated by High-throughput sequencing analysis, in triplicate, using illumina HiSeq 2000. And the differential mRNA profiles were analyzed. Results: mRNA profiles of more than 20000 genes were analyzed, and differential expression profiles were compared . Conclusions: Our study represents the first detailed analysis of transcriptomes of WT and Kcnj2 KO mouse BMDM treated with LPS or kir2.1 inhibitor, with biologic replicates, generated by RNA-seq technology. This data was useful to analyze the relationship between Kir2.1 and LPS induced innate immune respone

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