Project description:Background: Salmonid species have followed markedly divergent evolutionary trajectories in their interactions with sea lice. While sea lice parasitism poses significant economic, environmental, and animal welfare challenges for Atlantic salmon (Salmo salar) aquaculture, coho salmon (Oncorhynchus kisutch) exhibit near-complete resistance to sea lice, achieved through a potent epithelial hyperplasia response leading to rapid louse detachment. The molecular mechanisms underlying these divergent responses to sea lice are unknown. Results: We characterised the cellular and molecular responses of Atlantic salmon and coho salmon to sea lice using single-nuclei RNA sequencing. Juvenile fish were exposed to copepodid sea lice (Lepeophtheirus salmonis), and lice-attached pelvic fin and skin samples were collected 12h, 24h, 36h, 48h, and 60h after exposure, along with control samples. Comparative analysis of control and treatment samples revealed an immune and wound-healing response that was common to both species, but attenuated in Atlantic salmon, potentially reflecting greater sea louse immunomodulation. Our results revealed unique but complementary roles of three layers of keratinocytes in the epithelial hyperplasia response leading to rapid sea lice rejection in coho salmon. Our results suggest that basal keratinocytes direct the expansion and mobility of intermediate and, especially, superficial keratinocytes, which eventually encapsulate the parasite. Conclusions: Our results highlight the key role of keratinocytes in coho salmon’s sea lice resistance, and the diverged biological response of the two salmonid host species when interacting with this parasite. This study has identified key pathways and candidate genes that could be manipulated using various biotechnological solutions to improve Atlantic salmon sea lice resistance.
Project description:In the present study, we studied microbial composition and metabolic activity in the bathypelagic zone of the South China Sea. 12 samples were collected and subjected to metaproteomic analysis. Our data provide a novel view of the roles of two lifestyle prokaryotes and their link in substrate utilization in dark ocean.
Project description:In the present study, we studied microbial composition and metabolic activity in the euphotic zone of the South China Sea. 8 samples were collected and subjected to metaproteomic analysis. Our results suggested that mixotrophic phototrophs-driven NDL carbon fixation along with phytoplankton-driven NRL carbon fixation determined primary production in the oligotrophic ocean’s euphotic zone.
2024-06-16 | PXD018513 | Pride
Project description:Microbiota of some coral species living in the Sea of Marmara
Project description:Interventions: Group 1: Arm 1: Blood-, urine- and stool-samples of 60 patients with gastrointestinal tumors (UICC III-IV) without tumor cachexia will be frozen. The samples will be collected and send to the analysis lab for analysis. In addition, a questionnaire will be completed by the patient.
Group 2: Arm 2: Blood-, urine- and stool-samples of 60 patients with gastrointestinal tumors (UICC III-IV) with tumor cachexia will be frozen. The samples will be collected and send to the analysis lab for analysis. In addition, a questionnaire will be completed by the patient.
Primary outcome(s): - Evaluation of intestinal genes in stool samples of patients using next-generation sequencing technology
- Identification of differences regarding the diversity of intestinal microorganisms between both groups
Primary and endpoint will be assessed once based on the collected samples. No follow-up samples will be collected.
Study Design: Allocation: ; Masking: ; Control: ; Assignment: ; Study design purpose: basic science