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METTL14 is a chromatin regulator independent of RNA N6-methyladenosine (nuclear RNA)

ABSTRACT: METTL14 is a chromatin regulator independent of RNA N6-methyladenosine (nuclear RNA)

PROVIDER: PRJNA760513 | ENA |

REPOSITORIES: ENA

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METTL14 is a chromatin regulator independent of RNA N6-methyladenosine

Project description:METTL14 is a chromatin regulator independent of RNA N6-methyladenosine

| PRJNA760507 | ENA

METTL14 is a chromatin regulator independent of RNA N6-methyladenosine (mRNA)

Project description:METTL14 is a chromatin regulator independent of RNA N6-methyladenosine (mRNA)

| PRJNA760511 | ENA

METTL14 is a chromatin regulator independent of RNA N6-methyladenosine (ChIP-Seq)

Project description:METTL14 is a chromatin regulator independent of RNA N6-methyladenosine (ChIP-Seq)

| PRJNA760504 | ENA

METTL14 is a chromatin regulator independent of RNA N6-methyladenosine (CUT & RUN)

Project description:METTL14 is a chromatin regulator independent of RNA N6-methyladenosine (CUT & RUN)

| PRJNA760503 | ENA

METTL14 is a chromatin regulator independent of RNA N6-methyladenosine (nascent tRNA)

Project description:METTL14 is a chromatin regulator independent of RNA N6-methyladenosine (nascent tRNA)

| PRJNA760512 | ENA

Widespread N6-methyladenosine-dependent RNA Structural Switches Regulate RNA-Protein Interactions

Project description:We show that N6-methyladenosine (m6A), the most abundant internal modification in mRNA/lncRNA with still poorly characterized function, alters RNA structure to facilitate the access of RBM for heterogeneous nuclear ribonucleoprotein C (hnRNP C). We term this mechanism m6A-switch. Through combining PAR-CLIP with Me-RIP, we identify 39,060 m6A-switches among hnRNP C binding sites transcriptome-wide. We show that m6A-methyltransferases METTL3 or METTL14 knockdown decreases hnRNP C binding at 16,582 m6A-switches. Taken together, 2,798 m6A-switches of high confidence are identified to mediate RNA-hnRNP C interactions and affect diverse biological processes including cell cycle regulation. These findings reveal the biological importance of m6A and provide insights into the sophisticated regulation of RNA-RBP interactions through m6A-induced RNA structural remodeling. Measure the m6A methylated hnRNP C binding sites transcriptome-wide by PARCLIP-MeRIP; measure the differential hnRNP C occupancies upon METTL3/METTL14 knockdown by PAR-CLIP; measure RNA abundance and splicing level changes upon HNRNPC, METTL3 and METTL14 knockdown

2015-02-27 | E-GEOD-56010 | biostudies-arrayexpress

METTL14 is a chromatin regulator independent of RNA N6-methyladenosine (mRNA)

Project description:METTL3 and METTL14 are two components that form the core heterodimer of the main RNA m6A methyltransferase complex (MTC, also known as m6A writer) that installs m6A. Surprisingly, depletion of METTL3 or METTL14 displayed distinct effects on mouse embryonic stem cell (mESC) self-renewal. While comparable global hypo-methylation in RNA m6A was observed in Mettl3 or Mettl14 knockout mESCs, respectively. Mettl14 knockout led to a globally decreased nascent RNA synthesis, whereas Mettl3 depletion resulted in transcription upregulation, suggesting that METTL14 might possess an m6A-indenepent role in gene regulation. We found that METTL14 colocalizes with the repressive H3K27me3 modification and PRC2 complex. Mechanically, METTL14, but not METTL3, recognizes H3K27me3 and recruits KDM6B to induce H3K27me3 demethylation independent of METTL3. Depletion of METTL14 thus led to a global increase in H3K27me3 level along with a global gene suppression . The regulation of H3K27me3 by METTL14 is essential to the transition of mESCs from self-renewal to differentiation. This work reveals a regulation mechanism on heterochromatin by METTL14 in a manner distinct from METTL3 and independently of m6A, and critically impacts transcriptional regulation, stemness maintenance and differentiation of mESCs.

2024-08-16 | GSE183400 | GEO

Arginine methylation of METTL14 promotes RNA N6-methyladenosine modification

Project description:Both mRNA and proteins can be modified through addition of methyl groups. For example, addition of N6-methyladenosine (m6A) to mRNAs is critical for human development and health. Post-translational methylation of proteins can impact the dynamic regulation of enzymatic activity. Here we sought to explore the nexus of transcriptional and post-translational methylation by studying the role of methylation of the core methyltransferase METTL3/METTL14 in m6A regulation. We found by mass spectrometry that METTL14 arginine 255 (R255) is methylated (R255me). Global mRNA m6A levels were greatly decreased in METTL14 R255K mutant mouse embryonic stem cells (mESCs). We further found that R255me greatly enhances the interaction of METTL3/METTL14 with WTAP and promotes the binding of the complex to substrate RNA.

2021-05-14 | GSE164047 | GEO

METTL14 is a chromatin regulator independent of RNA N6-methyladenosine (nuclear RNA)

2024-08-16 | GSE183402 | GEO

METTL14 is a chromatin regulator independent of RNA N6-methyladenosine (m6A MeRIP-Seq)

Project description:METTL14 is a chromatin regulator independent of RNA N6-methyladenosine (m6A MeRIP-Seq)

| PRJNA760506 | ENA