Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Beyond the protoplast: isolating and analyzing fixed cells for plant single-cell research

ABSTRACT: Beyond the protoplast: isolating and analyzing fixed cells for plant single-cell research

PROVIDER: PRJNA760550 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15718200.fastq.gz	Fastqsanger.gz
	SRR15718300.fastq.gz	Fastqsanger.gz
	SRR15718400.fastq.gz	Fastqsanger.gz
	SRR15718201.fastq.gz	Fastqsanger.gz
	SRR15718301.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 303

Similar Datasets

Beyond the protoplast: isolating and analyzing fixed cells for plant single-cell research

Project description:We isolated fixed maize anther cells for single-cell RNA-seq. All anthers were 2.0 mm from the W23 inbred line.

2021-09-07 | GSE183422 | GEO

Protoplast data: suberized exodermis is required for tomato drought tolerance

Project description:This experiment aimed to identify protoplast-induced genes in tomato seedling, for future use in single cell datasets.

2023-10-01 | GSE212404 | GEO

transcriptome profiling from a protoplast culture of arabidopsis thaliana-From protoplast to plant : transcrip...

Project description:ra03-03_protoplats - transcriptome profiling from a protoplast culture of arabidopsis thaliana - transcriptome profiling and comparison of 6 status of differentiation - protoplasts were extracted from plantlet cultivated during 2 weeks then placed in a culture midium which stimulate cell division. Protoplasts were harvested after 0, 24, 48, 96 or 168 hours of culture. Biological repeat has been done (experiments A and C) Keywords: time course

2007-06-20 | GSE7984 | GEO

Isolating metastatic cell populations from zebrafish

Project description:Isolating metastatic cell populations from zebrafish

| PRJNA641651 | ENA

SnFFPE-Seq: towards scalable single nucleus RNA-seq of formalin-fixed paraffin-embedded (FFPE) tissues

Project description:Profiling cellular heterogeneity in formalin-fixed paraffin-embedded (FFPE) tissues is key to characterizing clinical specimens for biomarkers, therapeutic targets, and drug responses. Here, we optimize methods for isolating intact nuclei and single nucleus RNA-seq from FFPE tissues in the mouse brain, and demonstrate a pilot application to a human clinical specimen of lung adenocarcinoma. Our method opens the way to broad applications of snRNA-Seq to archival tissues, including clinical samples.

2022-08-31 | GSE211797 | GEO

Isolating S. mitis from nasopharyngeal carriage samples

Project description:Isolating S. mitis from nasopharyngeal carriage samples

| PRJEB53188 | ENA

Analysis of RNA quantitation in formaldehyde-fixed cells

Project description:Fluorescence-activated cell sorting (FACS) is a sensitive and valuable technique to characterize cellular subpopulations and great advances have been made using this approach. Cells are often fixed with formaldehyde prior to the sorting process to preserve cell morphology and maintain the expression of surface molecules, as well as to ensure safety in the sorting of infected cells. It is widely recognized that formaldehyde fixation alters RNA and DNA structure and integrity, thus analyzing gene expression in these cells has been difficult. We therefore examined the effects of formaldehyde fixation on the stability and quantitation of nucleic acids in cell lines, primary leukocytes and also cells isolated from SIV-infected pigtailed macaques. We developed a method to extract RNA from fixed cells that yielded the same amount of RNA as our common method of RNA isolation from fresh cells. Quantitation of RNA by RT-qPCR in fixed cells was not always comparable with that in unfixed cells. In comparison, when RNA was measured by the probe-based NanoString system, there was no significant difference in RNA quantitation. In addition, we demonstrated that quantitation of proviral DNA in fixed cells by qPCR is comparable to that in unfixed cells when normalized by a single-copy cellular gene. These results provide a systematic procedure to quantitate gene expression in cells that have been fixed with formaldehyde and sorted by FACS.

2013-09-09 | GSE50690 | GEO

Analysis of RNA quantitation in formaldehyde-fixed cells

2013-09-09 | E-GEOD-50690 | biostudies-arrayexpress

Dyspigmented hypertrophic scars: Beyond skin color

Project description:Dyspigmented hypertrophic scars: Beyond skin color

| PRJNA664128 | ENA

ANALYZING SOMATIC MUTAGENESIS IN SYSTEMIC SCLEROSIS

Project description:ANALYZING SOMATIC MUTAGENESIS IN SYSTEMIC SCLEROSIS

| PRJNA1131020 | ENA