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Exiguobacterium sibiricum strain:K1 | isolate:K1

ABSTRACT: Exiguobacterium sibiricum strain:K1 | isolate:K1 Genome sequencing

PROVIDER: PRJNA761004 | ENA |

REPOSITORIES: ENA

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Architecture of thermal adaptation in Exiguobacterium sibiricum strain 255-15: A genome and transcriptome approach

Project description:Several microorganisms have wide temperature growth range and versatility to tolerate large thermal fluctuations in diverse environments. To better understand thermal adaptation of psychrotrophs, Exiguobacterium sibiricum strain 255-15 was used, a psychrotrophic bacterium that grows from -5°C to 39°C. Its genome is approximately 3 Mb in size, has a GC content of 47.7% and includes 2,978 putative protein-encoding genes (CDS). The genome and transcriptome analysis along with the organism's known physiology was used to better understand its thermal adaptation. A total of about 27%, 3.2% and 5.2% of E. sibiricum strain 255-15 CDS spotted on the DNA microarray yielded differentially expressed genes in cells grown at -2.5°C, 10°C and 39°C, respectively, when compared to cells grown at 28°C. The hypothetical and unknown genes represented 10.6%, 0.89% and 2.3% of the CDS differentially expressed when grown at -2.5°C, 10°C and 39°C versus 28°C. The transcriptome analyses showed that E. sibiricum is constitutively adapted to cold temperatures since little differential gene expression was observed at growth temperatures of 10°C and 28°C, but at the extremities of its Arrhenius growth profile, namely -2.5°C and 39°C, much more differential gene expression occurred. The genes that responded were more typically associated with stress response. Keywords: stress response to cold and hot temperatures

2008-02-11 | GSE10133 | GEO

Architecture of thermal adaptation in Exiguobacterium sibiricum strain 255-15: A genome and transcriptome approach

Project description:Several microorganisms have wide temperature growth range and versatility to tolerate large thermal fluctuations in diverse environments. To better understand thermal adaptation of psychrotrophs, Exiguobacterium sibiricum strain 255-15 was used, a psychrotrophic bacterium that grows from -5Â°C to 39Â°C. Its genome is approximately 3 Mb in size, has a GC content of 47.7% and includes 2,978 putative protein-encoding genes (CDS). The genome and transcriptome analysis along with the organism's known physiology was used to better understand its thermal adaptation. A total of about 27%, 3.2% and 5.2% of E. sibiricum strain 255-15 CDS spotted on the DNA microarray yielded differentially expressed genes in cells grown at -2.5Â°C, 10Â°C and 39Â°C, respectively, when compared to cells grown at 28Â°C. The hypothetical and unknown genes represented 10.6%, 0.89% and 2.3% of the CDS differentially expressed when grown at -2.5Â°C, 10Â°C and 39Â°C versus 28Â°C. The transcriptome analyses showed that E. sibiricum is constitutively adapted to cold temperatures since little differential gene expression was observed at growth temperatures of 10Â°C and 28Â°C, but at the extremities of its Arrhenius growth profile, namely -2.5Â°C and 39Â°C, much more differential gene expression occurred. The genes that responded were more typically associated with stress response. Keywords: stress response to cold and hot temperatures Six-condition experiment: -2.5Â°C vs10Â°C, -2.5Â°C vs 28Â°C, -2.5Â°C vs 39Â°C, 28Â°C vs10Â°C, 28Â°C vs 39Â°C, 10Â°C vs 39Â°C. Biological replicates: 6 replicates grown and harvested independently for each different temperature (-2.5Â°C, 10Â°C, 28Â°C and 39Â°C). One replicate per array.

2010-05-26 | E-GEOD-10133 | biostudies-arrayexpress

Exiguobacterium sp. K1 strain:K1

Project description:Exiguobacterium sp. K1 strain:K1 Genome sequencing and assembly

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Exiguobacterium sibiricum NL255

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Exiguobacterium sibiricum 255-15 isolate:DSM 17290

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Exiguobacterium sibiricum 7-3

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Exiguobacterium sibiricum 255-15

Project description:Architecture of thermal adaptation in Exiguobacterium sibiricum strain 255-15: A genome and transcriptome approach

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Bacillus sp. K1 strain:k1 | isolate:k1

Project description:Bacillus sp. K1 strain:k1 | isolate:k1 Genome sequencing

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Comparison of developmental stage transcripts in the K1 strain

Project description:To identify the developmentally regulated genes, which could confound identification of PN and CQ drug responsive genes, RNA samples from drug-free synchronized cultures from ring, trophozoite, and schizont stages were individually labelled and hybridized with a pooled sample from the three stages. The data from this experiment were used to compare the developmental profile of the K1 strain with the data from other P. falciparum strains.

2011-08-02 | GSE30869 | GEO

Exiguobacterium acetylicum strain:SI17 | isolate:SI17

Project description:Exiguobacterium acetylicum strain:SI17 | isolate:SI17 Genome sequencing

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