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ChIP-seq of HNF1B in prostate cancer cell line DU145

ABSTRACT: ChIP-seq of HNF1B in prostate cancer cell line DU145

PROVIDER: PRJNA761335 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15736856_1.fastq.gz	Fastqsanger.gz
	SRR15736856_2.fastq.gz	Fastqsanger.gz
	SRR15736857_1.fastq.gz	Fastqsanger.gz
	SRR15736857_2.fastq.gz	Fastqsanger.gz

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ChIP-seq of HNF1B in prostate cancer cell line DU145

Project description:We report that HNF1B primarily bind promoter regions across the whole genome.

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Project description:DU145 prostate cancer cells were treated with 25 ng/ml hepatocyte growth factor (HGF) or vehicle for 2, 8, or 24 hours. HGF stimulates the cMET protein, a tyrosine kinase transmembrane protein. The aim of this study is to determine the role of the HGF/cMET pathway in immature cells of established prostate cancer. HGF stimulation of DU145 prostate cancer cell line led to cell migration in culture, formation of sprouts in Matrigel and inhibition of growth. These biological effects went together with induction of a stem-like phenotype as defined by up-regulation of CD49b, CD49f, CD44 and SOX9, and down-regulation of CD24 on gene-expression arrays and quantitative PCR. The shift towards a stem-like phenotype was reflected by protein modifications on FACS, Western blot, and enhanced rapid adhesion to collagen I. Small molecules SU11274 and PHA665752 were able to inhibit both morphologic and molecular HGF effects.

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Expression data of HGF/cMET pathway in prostate cancer DU145 cell line

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Comparative analysis of gene expression of cell line DU145 grown under sphere forming condition and as monolayer

Project description:By employing a global gene expression profiling, we performed analysis of the molecular pathways which are deregulated in prostate cancer cell line DU145 grown under tumorsphere forming condition and as standard attached monolayer culture. Both oriignal DU145 and its radioresistant derivative DU145-RR were analyzed

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Project description:Transcriptome analysis of prostate cancer patient derived organoid DU145 cell line upon knockdown of YAP, TAZ, or YAP/TAZ mediated by siRNAs

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FoxA1 inhibits androgen receptor expression and suppresses prostate cancer metastasis [DU145, ChIP-seq]

Project description:FoxA1 has been shown critical for prostate development and prostate-specific gene expression regulation. In addition to its well-established role as an AR pioneering factor,several studies have recently revealed significant AR binding events in prostate cancer cells with FoxA1 knockdown. Furthermore, the role of FoxA1 itself in prostate cancer has not been carefully examined. Thus, it is important to understand the role of FoxA1 in prostate cancer and how it interacts with AR signaling. ChIP-Seq examination of AR and FoxA1 binding sites, FAIRE-seq detection of open chromatin genomic regions in DU145 AR +/- FOXA1 cells

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Identification of eIF4A1-binding RNAs in human prostate cancer cell line DU145

Project description:Identification of eIF4A1-binding RNAs in human prostate cancer cell line DU145

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HNF1B VCaP ChIP-seq

Project description:ChIP-seq data for HNF1B VCaP and input.

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MicroRNA expression in DU145 sublines metastatic to lymph nodes

Project description:Comparison of non-coding RNA profiling by array in sublines of DU145 human prostate cancer cell lines created by in vivo cycling Cell lines created by removal and growth of metastatic human DU145 tumor cells from mouse lymph node (metastasized from prostate xenograft) for LN cells and extracted from lung after intravenous injection (ivLU cells). Cell line numebr represented number of in vivo cycles of metastatic selection

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Project description:Prostate cancer stem-like cells were derived from DU145 cells as suspension spheres. DU145 cells were transduced with a CNTN1 over expressing retroviral vector and a control empty vector. DU145 spheres were transduced using a retroviral-based shRNA vector against CNTN1 and a scrambled control shRNA viral vector. Both cell lines were cultured over a couple of passages before RNA was collected.

2016-02-12 | E-MTAB-4118 | biostudies-arrayexpress

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