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Gene expression in human keratinocytes cultivated with serum-free medium and serum containing medium

ABSTRACT: Gene expression in human keratinocytes cultivated with serum-free medium and serum containing medium

PROVIDER: PRJNA761384 | ENA |

REPOSITORIES: ENA

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Gene expression in human keratinocytes cultivated with serum-free medium and serum containing medium

Project description:The objective of this study was thus to develop ab initio that chemically defined serum-free medium. We used a statistical design of experiment (DOE) approach to screen a large number of candidate factors, for both their potential individual and synergetic effects, as to identify a formulation which was then tested extensively. In this study, we present the properties of keratinocyte cultivated with serum-Free medium (Surge SFM) and complemented DME-Ham medium (with serum).

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Characterization of human dental pulp cells grown in chemically defined serum-free medium

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RNA-seq of Mycoplasma pneumoniae grown in serum-free medium

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Gene Expression Profile of Human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) Culutured in Chemical Defined Serum Free Medium

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Project description:Differential expression analysis of mRNAs and proteins showed that the expression of genes related with apoptosis, immune response, and inflammatory response were significantly up-regulated in ADSCs cultivated in FBS containing media.

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Project description:This study describes the epigenetic and transcriptome profiling of mouse ES cells cultures in 2i serum-free medium. The proteins for which ChIP-Seq profiles were generated in these cells, as well as for cells grown in serum conditions, include H3K4me3, H3K36me3, H3K27me3, H3K9me3, Suz12 and Ezh2. A sequence profile of genomic DNA is also included. Furthermore, we included RNA-Seq of ds_cDNA synthesized from double poly(A) selected mRNA and from rRNA depleted (strand specific) total RNA.

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Gene Expression Profile of Human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) Culutured in Chemical Defined Serum Free Medium

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