Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from autophagy-deficient keratinocytes and normal keratinocytes

ABSTRACT: Expression data from autophagy-deficient keratinocytes and normal keratinocytes

PROVIDER: PRJNA762339 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Expression data from autophagy-deficient keratinocytes and normal keratinocytes

Project description:There is increasing evidence that autophagy contributes to the epidermal differentiation; however, the role of autophagy in epidermal tight junction (TJ) barrier remains unclear. To evaluate the role of autophagy in the maintenance of skin TJ barrier, we knocked out autophagy in human primary keratinocytes by infecting cells with autophagy-related gene 3 (Atg3) C264S mutant adenovirus.

2022-07-14 | GSE183921 | GEO

Autophagy deficient keratinocytes under paraquat stress

Project description:Autophagy is a mechanism that regulates cellular metabolism and clearance of damaged macromolecules and organelles. Impaired degradation of modified macromolecules contributes to cellular dysfunction and is observed in aged tissue and senescent cells. We have inactivated Atg7, an essential autophagy gene, in murine keratinocytes and have found in an earlier study that this resulted in increased baseline oxidative stress and reduced capacity to degrade crosslinked proteins after oxidative ultraviolet stress. To investigate whether autophagy deficiency would promote cellular aging, we studied, how Atg7 deficient (KO) and Atg7 bearing cells (WT) would respond to stress induced by Paraquat (PQ), an oxidant drug commonly used to induce cellular senescence.

2017-02-14 | GSE84935 | GEO

Expression data from primary keratinocytes obtained from WT and keratinocyte specific Glut1-deficient mice [MTA-1_0]

Project description:Glut1 is highly expressed in basal cells of keratinocytes, but the regulation of Glut1 and expression of additional glucose transporters in the skin has not been explored, here we specifically ablate Glut1 in epidermal keratinocytes to elucidate the role of glucose transport in the keratinocytes. we performed microarray analysis in WT and Glut1 deficient primary keratinocytes to determine the pathways might contribute to the impaired proliferation in Glut1 deficient keratinocytes.

2018-01-01 | GSE94884 | GEO

Autophagy controls the protein composition of hair shafts

Project description:Hair shafts are formed by terminal differentiation of hair keratinocytes (trichocytes) in which keratins and keratin-associated proteins accumulate and undergo cross-linking. Here we tested the hypothesis that maturation of the hair shaft also involves the coordinated degradation of other proteins and, specifically, that this degradation is mediated by autophagy. To this end, we deleted the non-redundant autophagy regulator Atg7 in keratinocytes of the epidermis and skin appendages, including hair, and determined the proteome of hair shafts from fully autophagy-competent and epithelial autophagy-deficient mice. The abrogation of autophagy led to significantly increased abundance of proteins regulating house-keeping functions of the cell and a decrease of cytoskeletal proteins. Translation factors, tRNA-ligases, ribosomal proteins and the components of proteasomes were particularly elevated in the absence of autophagy, indicating a central role of autophagy in regulating multiple steps of protein turnover in hair keratinocytes. These results demonstrate that hair keratinocytes depend on autophagy for establishing the mature protein composition of hair.

2023-01-17 | MSV000091072 | MassIVE

Chromatin-bound proteins in autophagy deficient cells

Project description:To determine the role of autophagy in the maintenance of genome stability and nucleic acid metabolism, the chromatin-bound proteins in autophagy-deficient ATG7-/- HEK293 cells were compared with autophagy-proficient ATG7+/+ HEK293 cells by Data-independent acquisition mass spectrometry (DIA-MS).

2021-10-27 | PXD028956 | JPOST Repository

Normal and Cancer Exosomes Induced Gene Expression Profiles in Primary Human Normal Oral Keratinocytes

Project description:Gene expression reprogramming in normal oral keratinocytes following exposure to normal or cancer-derived exosomes.

2018-07-16 | GSE89217 | GEO

Expression data between migrating keratinocytes in diabetic mice against normal mice during re-epithelialization 3 days post wounding

Project description:During re-epithelialization, keratinocytes have to undergo specific gene expression changes that grant them increased motility. Diabetic keratinocytes have been observed to portray an impairment in this aspect. We used microarrays to identify the changes in the expression patterns of both normal and diabetic migrating keratinocytes during re-epithelialization.

2020-10-23 | GSE141956 | GEO

Adenosine stimulation of normal human epidermal keratinocytes (NHEK)

Project description:Adenosine stimulation of normal human epidermal keratinocytes (NHEK)

| PRJNA693696 | ENA

Adenosine stimulation of normal human epidermal keratinocytes (NHEK)

Project description:Gene expression profile of normal human epidermal keratinocytes treated with adenosine

2021-01-22 | GSE165222 | GEO

Expression data from keratinocytes of WT and RASSF9-/- mice

Project description:Mice with deficient expression of RASSF9 exhibit intriguing phenotypes of skin-related pathology, including abnormal thickening of the epidermis, dysregulated proliferation of keratinocytes, and alopecia. To delineate the underlying mechanism, we profiled gene expression in keratinocytes of RASSF9-mutant mice to identify targets whose expressions were affected by RASSF9 gene deletion.

2010-09-17 | GSE24190 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data