Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Epigenome analysis of gliomas with EWSR1-BEND2 fusions

ABSTRACT: Epigenome analysis of gliomas with EWSR1-BEND2 fusions

PROVIDER: PRJNA762556 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Epigenome analysis of gliomas with EWSR1-BEND2 fusions

Project description:Genome-wide DNA methylation profiling of 4 gliomas with EWSR1-BEND fusions with histologic features resembling astroblastoma. The Illumina Infinium EPIC 850k Human DNA Methylation Beadchip was used to obtain DNA methylation profiles across approximately 850,000 CpG sites of genomic DNA extracted from formalin-fixed, paraffin-embedded tumor tissue of 4 gliomas with EWSR1-BEND2 fusions.

2021-11-18 | GSE183972 | GEO

Epigenome analysis of human brain gliomas

Project description:Epigenome analysis of human brain gliomas

| PRJNA231171 | ENA

Expression data from Sarcoma Tumors expressing the EWSR1-CREB fusions

Project description:Expression data from Sarcoma Tumors expressing the EWSR1-CREB fusions

| PRJNA708292 | ENA

Expression data from Sarcoma Tumors expressing the EWSR1-CREB fusions [microarray]

Project description:Expression data from Sarcoma Tumors expressing the EWSR1-CREB fusions [microarray]

| PRJNA708295 | ENA

Epigenome analysis of pediatric bithalamic diffuse gliomas

Project description:Epigenome analysis of pediatric bithalamic diffuse gliomas

| PRJNA588372 | ENA

Expression data from Sarcoma Tumors expressing the EWSR1-CREB fusions [microarray]

Project description:Sarcoma is a rare form of tumor characterized by the presence of oncogenic fusion, that is often the only aberration identified. U133A Affymetrix arrays were used to identify the expression signature of 3 different types of sarcoma: AFH, CCS and GI-CCS harboring the EWSR1-CREB1 and EWSR1-ATF1 chromosomal translocation.

2021-08-18 | GSE168560 | GEO

Multi-Omic and Functional Analysis for Classification and Treatment of Sarcomas with FUS-TFCP2 or EWSR1-TFCP2 Fusions

Project description:Linking clinical multi-omics analyses with mechanistic studies may improve the understanding of rare cancers. We leveraged two precision oncology programs to investigate rhabdomyosarcoma with FUS/EWSR1-TFCP2 fusions, an orphan malignancy without effective therapies. All tumors exhibited outlier ALK expression, partly accompanied by intragenic deletions and aberrant splicing. This resulted in the expression of ALK variants, i.e. short transcripts (ST), which we named ALK-ST1 (consisting of exons 1-2:18–29), ALK-ST2 (1:18–29), ALK-ST3 (18–29), and ALK-ST4 (1–5:12–17). To systematically investigate the oncogenic capacity of these ALK variants, we stably expressed them in p53-deficient MCF10A human mammary epithelial cells and performed different transformation assays. These experiments demonstrated that ALK-ST1, ALK-ST2, and ALK-ST3 are oncogenic variants, while ALK-ST4 could not transform MCF10A cells. We confirmed protein expression of ALK-ST1, ALK-ST2, and ALK-ST3 by western blotting, which was not possible for ALK-ST4 due to lack of a specific antibody that binds to the N-terminus of ALK that is lost in ALK-ST4. We therefore performed mass spectrometry-based label-free quantitative proteomics on lysates from MCF10A cells stably expressing empty vector (EV), wildtype ALK (ALK-WT), or ALK-ST4 to confirm its expression.

2023-11-13 | PXD045522 | Pride

EWSR1-BEND2 fusion defines an epigenetically distinct subtype of astroblastoma.

Project description: Not available

| S-EPMC8732961 | biostudies-literature

Epigenome analysis of intracranial mesenchymal tumors with FET-CREB fusions

Project description:Epigenome analysis of intracranial mesenchymal tumors with FET-CREB fusions

| PRJNA692742 | ENA

Epigenome analysis of low-grade neuroepithelial tumors with FGFR2 fusions

Project description:Epigenome analysis of low-grade neuroepithelial tumors with FGFR2 fusions

| PRJNA721967 | ENA