Project description:Transcriptional profiling of human colorectal cancer tissues comparing control from histologically normal tissue samples adjacent to the tumors. High throughput bioluminescence imaging, and PET were performed. The overall goal was to determine the differential expression of lncRNA and mRNA between tumor and match normal samples.
Project description:Genomic DNA samples were extracted from microdissected colorectal tumour tissue and then compared against normal DNA extracted from the peripheral blood from the same patients. 53 Normal/Tumor matched samples.
Project description:Next-generation sequencing (NGS) has revolutionized systems-based analysis of gene expression. The goals of this study is to compare gene expressions in colorectal tumor versus adjacent normal colorectal tissue.
Project description:We performed gene expression profiling of 26 colorectal tumors and matched histologically normal adjacent colonic tissue samples using the Illumina Ref-8 whole-genome expression BeadChip. We performed an integrated analysis of promoter DNA methylation and gene expression data to investigate the effects of DNA hypermethylation on gene expression. Total RNA was isolated from 26 pairs of fresh frozen colorectal tumor and matched adjacent non-tumor tissue samples. Their gene expression profiles were obtained using the Illumina Ref-8 whole-genome expression BeadChip (HumanRef-8 v3.0, 24,526 transcripts).
Project description:Genomic DNA samples were extracted from microdissected colorectal tumour tissue and then compared against normal DNA extracted from the peripheral blood from the same patients.
Project description:We evaluated the presence of genome-wide epigenetic differences at 385,000 loci using Encode HG18 DNA methylation arrays in 5 non-tumor-associated (NTA) and 4 histologically undistinguished tumor-associated (TA) prostate tissues. We identified 615 probes to be differentially methylated (p<0.05) in TA prostate, with 537 (87%) hypomethylated and 78 (13%) hypermethylated. comparison of methylation in NTA to TA prostate tissue
Project description:Analysis of 4 pairs of colorectal cancer tissue and paraneoplastic tissue. The fresh frozen samples were obtained during surgical procedures. Results provide insight into molecular mechanisms underlying malignancy.
Project description:Analysis of differentially regulated genes in patients normal and colorectal tumor samples. The aim of this study was to identify genes that were differentially up- or down-regulated in the tumor samples as compared to the normal colon tissue