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Genome-wide transcriptome analysis of pluripotent (2iL) and differentiated (2iL withdrawal) mES cells, overexpressing a DOX-inducible Esrrb

ABSTRACT: Genome-wide transcriptome analysis of pluripotent (2iL) and differentiated (2iL withdrawal) mES cells, overexpressing a DOX-inducible Esrrb

PROVIDER: PRJNA763238 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15900287.fastq.gz	Fastqsanger.gz
	SRR15900288.fastq.gz	Fastqsanger.gz
	SRR15900289.fastq.gz	Fastqsanger.gz
	SRR15900290.fastq.gz	Fastqsanger.gz
	SRR15900291.fastq.gz	Fastqsanger.gz

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Genome-wide transcriptome analysis of pluripotent (2iL) and differentiated (2iL withdrawal) mES cells, overexpressing a DOX-inducible Esrrb

Project description:We report the application of genome-wide RNA-sequencing analysis for pluripotent mouse ES cells transfected with DOX-inducible Esrrb and maintained in 2iL, or differentiated for 96 hours with or without Doxycycline. We find that Esrrb expression is sufficient to confer reversibility and activates both naive and formative genes.

2023-01-25 | GSE184132 | GEO

Genome-wide transcriptome analysis of pluripotent (2iL) or differentiated (2iL withdrawal) wild-type or Esrrb KO mES cells.

Project description:Genome-wide transcriptome analysis of pluripotent (2iL) or differentiated (2iL withdrawal) wild-type or Esrrb KO mES cells.

| PRJNA763237 | ENA

Genome-wide transcriptome analysis of pluripotent (2iL) or differentiated (2iL withdrawal) wild-type or Esrrb KO mES cells.

Project description:We report the application of genome-wide RNA-sequencing analysis for pluripotent wild-type or Esrrb KO mouse ES cells, maintained in 2iL or differentiated for 24, 48 or 72 hours. We find an anticipated progression of Esrrb KO cells as compared to wild-type cells and a massive downregulation of formative genes in multiple Esrrb KO clones, confirming the role of Esrrb as an inducer of the formative program. Such Esrrb KO cells also show upregulation of committed genes and activation of the Mesendoderm program.

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Genome-wide maps of ESRRB binding in pluripotent (2iL) and differentiated (48h, 96h) mES cells

Project description:We report the application of sequencing technology for profiling of genome-wide ESRRB binding in mouse ES cells. We generated genome-wide chromatin-state maps of mouse embryonic stem cells maintained in pluripotent state (2iL) and differentiated for 48 and 96 hours (48 and 96). We find that the large majority of ESRRB binding occurs in 2iL and at 48 hours and that several formative genes promoters are bound by ESRRB in 2iL and at 48h. Moreover ESRRB peaks on naïve genes (e.g. Tfcp2l1, Nanog) decrease as soon as cells start differentiating while peaks on formative genes are present in 2iL and the signal is maintained (or increased) at 48 hours, further endorsing the concept of Esrrb as a direct activator of the formative gene program during the Reversible phase.

2023-01-25 | GSE184131 | GEO

Genome-wide transcriptome analysis of pluripotent (2iL), differentiated (2iL withdrawal) or re-induced (2iL re-application during differentiation) mES cells

Project description:Genome-wide transcriptome analysis of pluripotent (2iL), differentiated (2iL withdrawal) or re-induced (2iL re-application during differentiation) mES cells

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Genome-wide maps of ESRRB binding in pluripotent (2iL) and differentiated (48h, 96h) mES cells

Project description:Genome-wide maps of ESRRB binding in pluripotent (2iL) and differentiated (48h, 96h) mES cells

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Genome-wide transcriptome analysis of pluripotent (2iL), differentiated (2iL withdrawal) or re-induced (2iL re-application during differentiation) mES cells

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2023-01-25 | GSE184134 | GEO

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