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ABSTRACT: PE film residue

PROVIDER: PRJNA764183 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15988900_1.fastq.gz	Fastqsanger.gz
	SRR15988900_2.fastq.gz	Fastqsanger.gz
	SRR15988901_1.fastq.gz	Fastqsanger.gz
	SRR15988901_2.fastq.gz	Fastqsanger.gz
	SRR15988902_1.fastq.gz	Fastqsanger.gz

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Project description:We have developed a novel in vitro protocol for the derivation of bona fide Pharyngeal Endoderm (PE) cells from hESCs. We demonstrated that our PE cells robustly express Pharyngeal Endoderm markers, they are transcriptionally similar to PE cells isolated from in vivo mouse development and represent a transcriptionally homogeneous population. Importantly, we elucidated the contribution of Retinoic Acid in promoting a transcriptional and epigenetic rewiring of PE cells. In addition, we defined the epigenetic landscape of PE cells by combining ATAC-Seq and ChIP-Seq of histone marks.

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NEXT GENERATION SEQUENCING DETECTS DYSREGULATED PLACENTAL MICRORNAS IN PREECLAMPSIA

Project description:Introduction: To determine the miRNA expression profile in pregnancies complicated by Preeclampsia (PE) and compare it to uncomplicated pregnancies. Methods: Seventeen placentas from women with PE, [all of them being late onset PE (LOPE)], as well as 17 placentas from uncomplicated pregnancies were analyzed using miRNA NGS. For statistical analyses the MATLAB® simulation environment was applied. The expression of miR-99b and miR-23b were verified using Quantitative Real-Time Polymerase Chain Reaction. Results: Two miRNAs were found dysregulated in PE complicated placentas. Statistical analysis revealed that miR-99b and miR-23b were under-expressed in complicated placentas as compared to controls. Discussion: Since specific miRNAs can differentiate complicated from uncomplicated pregnancies, they may be considered as putative PE-specific biomarkers.

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