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Expression data from FedEV uptake mesothelial cells

ABSTRACT: Expression data from FedEV uptake mesothelial cells

PROVIDER: PRJNA764229 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15931771_1.fastq.gz	Fastqsanger.gz
	SRR15931771_2.fastq.gz	Fastqsanger.gz
	SRR15931772_1.fastq.gz	Fastqsanger.gz
	SRR15931772_2.fastq.gz	Fastqsanger.gz
	SRR15931773_1.fastq.gz	Fastqsanger.gz

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Expression data from FedEV uptake mesothelial cells

Project description:Asbestos-associated diseases remain a social burden worldwide. Our previous studies identified asbestos-induced iron-rich milieu for mesothelial cells with ceaseless macrophage ferroptosis. However, molecular mechanisms how this mutagenic milieu influences mesothelial cells have not been elucidated yet. Here, we propose a novel mechanism that extracellular vesicles (EVs) mediate asbestos-associated mutagenic factors to mesothelial cells. In a mice model of intraperitoneal crocidolite injection, mutagenic milieu highly expressed CD63, an exosomal marker. We then used a CD63-GFP labeled THP-1 macrophage model exposed to crocidolite/iron, which generated EVs under ferroptotic process. We observed that MeT-5A mesothelial cells can receive and internalize these EVs. Furthermore, we comprehensively analyzed the ferroptosis-dependent EVs (FedEVs) for transported proteins and identified ferritin heavy/light chains as major proteins. Therefore, we inferred that FedEVs transport iron from ferroptotic macrophages to mesothelial cells. RNA sequencing revealed that the mesothelial cells receiving higher amounts of the FedEVs were mitotic, especially at the S and G2/M phases, by the use of Fucci mesothelial cells. Nuclear 8-hydroxy-2`-deoxyguanosine and γ-H2AX were significantly increased in the recipient mesothelial cells after exposure to FedEVs. Collectively, we here demonstrate a novel mechanism that FedEVs act as a key mutagenic mediator by transporting iron, which contribute to asbestos-induced mesothelial carcinogenesis.

2021-10-22 | GSE184368 | GEO

Expression data of OVCAR8 cells with and without mesothelial cells

Project description:Expression data of OVCAR8 cells with and without mesothelial cells

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Expression data of OVCAR8 cells with and without mesothelial cells

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Expression data from fetal and adult mouse liver mesothelial cells

Project description:Characterization and Functional Analyses of Hepatic Mesothelial Cells in Mouse Liver Development. We used microarrays to detail the global programme of gene expression in mesothelial cells during mouse liver development. Mouse liver mesothelial cells were selected by a cell sorter at E12.5 and adult for RNA extraction and hybridization on Affymetrix microarrays. We obtained PCLP1+ cells and Mesothelin+ cells at each developmental stage in order to analyze expression profiles of mesothelial cells.

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Expression data from fetal and adult mouse liver mesothelial cells

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lncRNA expression alterations in mesothelial cells treated with exosomes

Project description:lncRNA expression alterations in mesothelial cells treated with exosomes

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Identification of mesothelial-to-mesenchymal gene signature in ascitic fluid-isolated mesothelial cells through RNA-sequencing

Project description:RNA-sequencing analysis was carried out on ascetic fluid-isolated mesothelial cells from ovarian cancer patients compared to control human peritoneal mesothelial cells to identify a mesothelial-mesenchymal gene signature.

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Project description:Bulk RNA-seq was performed on fibroblasts and mesothelial cells from 11 different healthy mouse tissues such as bone, epididymal, brown and inguinal fat pads, mesentery, omentum, pancreas, liver, lung, lymph node and spleen. This data was used to examine tissue specific gene expression programs of fibroblasts and mesothelial cells.

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Expression of genes in E17 p57-/- and p57+/+ hepatic mesothelial and submesothelial cells

Project description:To analyze p57 function in mouse fetal liver, we surgically isolated mesothelial and submesothelial cells from E17 p57-/- and p57+/+ livers. Compared gene expression in E17 p57-/- and p57+/+ hepatic mesothelial and submesothelial cells.

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RNA-seq gene expression profiles of primary murine peritoneal mesothelial cells.

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