Mex3a RIP-seq
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA764522 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR15965271.fastq.gz | Fastqsanger.gz | |||
| SRR15965272.fastq.gz | Fastqsanger.gz | |||
| SRR15965273.fastq.gz | Fastqsanger.gz | |||
| SRR15965274.fastq.gz | Fastqsanger.gz | |||
| SRR15965275.fastq.gz | Fastqsanger.gz |
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Project description:The effort to better understand intestinal stem cell (ISC) identity and regulation remains a challenge. We have been studying the RNA-binding protein MEX3A as a putative ISC marker. In that context, we have generated the first Mex3a knockout (KO) mouse model and show MEX3A is crucial for maintenance of the Lgr5+ ISC pool. As part of a phenotypic characterization pipeline, we have performed transcriptomic profiling (RNA-sequencing) of isolated Mex3a KO small intestinal crypts and compared it against small intestinal crypts isolated from age-matched wild-type controls.
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