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Mesenchymal cell type-specific roles of KLF4 in lung fibrosis [bulk RNA-seq]

ABSTRACT: Mesenchymal cell type-specific roles of KLF4 in lung fibrosis [bulk RNA-seq]

PROVIDER: PRJNA765610 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16024080_1.fastq.gz	Fastqsanger.gz
	SRR16024080_2.fastq.gz	Fastqsanger.gz
	SRR16024081_1.fastq.gz	Fastqsanger.gz
	SRR16024081_2.fastq.gz	Fastqsanger.gz
	SRR16024082_1.fastq.gz	Fastqsanger.gz

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Mesenchymal cell type-specific roles of KLF4 in lung fibrosis [bulk RNA-seq]

Project description:Purpose: The goal of this study is to investigate mesenchymal cell-type specific role of KLF4 in the context of lung fibrosis. Methods: Adult Pdgfrb-CreERT2, ROSA26R(Zs/+) mice were induced with tamoxifen (1mg/day for 5 days), rested for 5 days. The lungs were harvested and prepared to obtain single cell suspension. Live Zs+ cells were isolated by FACS, cultured, and subjected to Klf4 siRNA or Scr RNA treatment. Total RNA was purified after 72 hrs. and subjected to bulk RNA-seq. using Illumina HiSeq 2000. Similarly, wild type mice airway smooth muscle cells (cell biologics) were treated with Klf4 siRNA or Scr RNA. Total RNA was purified after 72 hrs. and subjected to bulk RNA-seq. Results: Expression profile of the transcriptome are compared between cell-types; PDGFR-β+ cells and airway smooth muscle cells. Conclusions: Significant difference in the expression of genes involved in pathogenesis of fibrosis was observed

2021-09-23 | GSE184670 | GEO

Mesenchymal cell type-specific roles of KLF4 in lung fibrosis

Project description:Mesenchymal cell type-specific roles of KLF4 in lung fibrosis

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Roles of circular RNA in cardiac fibrosis

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Single-cell RNA-sequencing reveals profibrotic roles of distinct epithelial and mesenchymal lineages in pulmonary fibrosis

Project description:Single-cell RNA-sequencing reveals profibrotic roles of distinct epithelial and mesenchymal lineages in pulmonary fibrosis

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Comprehensive bulk RNAseq analysis on whole kidneys during regeneration and fibrosis development reveals sustained dysregulation of immune system related pathways during kidney fibrosis

Project description:In order to identify the transcriptional changes that correlate with kidney regeneration or fibrosis development, we performed a time-course bulk RNA-seq from whole-kidneys at 3, 7, 14, 28 and 42 days after the initial ischemic kidney from two distinct murine models.

2020-01-31 | E-MTAB-7957 | biostudies-arrayexpress

Dysregulated mesenchymal PDGFR-β drives kidney fibrosis

Project description:Kidney fibrosis is characterized by expansion and activation of platelet-derived growth factor receptor-β (PDGFR-β) positive mesenchymal cells. To study the consequences of PDGFR-ß activation, we developed a model of primary renal fibrosis using transgenic mice with PDGFR-β activation specifically in renal mesenchymal cells, driving their pathological proliferation and phenotypic switch towards myofibroblasts. This resulted in progressive mesangioproliferative glomerulonephritis, mesangial sclerosis and interstitial fibrosis with progressive anemia due to loss of erythropoietin production by fibroblasts. We used microarrays to compare wildtype animals (Foxd1_wt Pdgfrb_wt) to animals with constitutive mesenchymal PDGFR-β activation (Foxd1_mt Pdgfrb V536A) in the kidney to identify target genes of PDGFR-β signaling.

2019-12-06 | GSE138819 | GEO

Mesenchymal cell type-specific roles of KLF4 in lung fibrosis

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-09-23 | GSE184672 | GEO

Dysregulated mesenchymal PDGFR-β drives kidney fibrosis

Project description:Dysregulated mesenchymal PDGFR-β drives kidney fibrosis

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KLF4-PFKFB3-driven glycolysis is essential for phenotypic switching of vascular smooth muscle cells

Project description:Vascular smooth muscle cells (VSMCs) within atherosclerotic lesions undergo a phenotypic switching in a KLF4-dependent manner. Glycolysis plays important roles in transdifferentiation of somatic cells, however, it is unclear whether and how KLF4 mediates the link between glycolytic switch and VSMCs phenotypic transitions. Here, we show that KLF4 upregulation accompanies VSMCs phenotypic switching in atherosclerotic lesions. KLF4 enhances the metabolic switch to glycolysis through increasing PFKFB3 expression. Inhibiting glycolysis suppresses KLF4-induced VSMCs phenotypic switching, demonstrating that glycolytic shift is required for VSMCs phenotypic switching. Mechanistically, KLF4 upregulates expression of circCTDP1 and eEF1A2, both of which cooperatively promote PFKFB3 expression. TMAO induces glycolytic shift and VSMCs phenotypic switching by upregulating KLF4. Our study indicates that KLF4 mediates the link between glycolytic switch and VSMCs phenotypic transitions, suggesting that a previously unrecognized KLF4-eEF1A2/circCTDP1-PFKFB3 axis plays crucial roles in VSMCs phenotypic switching.

2022-11-09 | PXD038057 |

Effects of ginkgetin on liver fibrosis by bulk RNA Sequencing

Project description:Ginkgetin could significantly attenuate liver fibrosis in a mouse model induced by TAA, including inhibiting hepatic inflammtion and fibrosis. In addition, the related signaling pathways and gene expression were also downregulated by ginkgetin by bulk RNA Sequencing analysis.

2023-08-03 | GSE236779 | GEO

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