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A flavonoid-attracted Aeromonas spp enhances plant drought resistance without cry-for-help [Plant_RNA-seq]

ABSTRACT: A flavonoid-attracted Aeromonas spp enhances plant drought resistance without cry-for-help [Plant_RNA-seq]

PROVIDER: PRJNA766686 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16090783_1.fastq.gz	Fastqsanger.gz
	SRR16090783_2.fastq.gz	Fastqsanger.gz
	SRR16090784_1.fastq.gz	Fastqsanger.gz
	SRR16090784_2.fastq.gz	Fastqsanger.gz
	SRR16090785_1.fastq.gz	Fastqsanger.gz

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A flavonoid-attracted Aeromonas spp enhances plant Na_H1 resistance without cry-for-help [Bacteria_RNA-seq]

Project description:A flavonoid-attracted Aeromonas spp enhances plant Na_H1 resistance without cry-for-help [Bacteria_RNA-seq]

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A flavonoid-attracted Aeromonas spp enhances plant drought resistance without cry-for-help [Plant_RNA-seq]

Project description:Flavonoids are stress-inducible metabolites important for plant-microbe interactions. In contrast to their well-known function in initiating rhizobia nodulation in legumes, it is unclear whether and how flavonoids may contribute to plant stress resistance through affecting non-nodulating bacteria in the root microbiome. Here we show how flavonoids preferentially attracts Aeromonadaceae in Arabidopsis thaliana root microbiome and how flavonoid-dependent recruitment of an Aeromona spp. results in enhanced plant drought resistance.

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A flavonoid-attracted Aeromonas spp enhances plant Na_H1 resistance without cry-for-help [Bacteria_RNA-seq]

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Project description:A flavonoid-attracted Aeromonas spp enhances plant drought resistance without cry-for help

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Circadian Clock Protein CRY Regulates Autoimmunity

Project description:The circadian system regulates numerous physiological processes including the adaptive immune system. Here we show that mice deficient for the circadian genes Cry1 and Cry2, (DKO) display an autoimmune phenotype including higher serum IgG concentration, presence of serum anti-nuclear antibodies, precipitation of IgG, IgM and complement 3 in glomeruli, and massive infiltrations of leukocytes into the lung and kidney. Activation of Cry DKO splenic B cells elicited markedly enhanced and prolonged tyrosine phosphorylation of cellular proteins compared to cells from control mice, suggesting that over activation of the BCR signaling pathway may contribute to autoimmunity in the Cry DKO mice. Expression of C1q, deficiency of which contributes to the pathogenesis of Systemic lupus erythematosus (SLE), was significantly downregulated in Cry DKO B cells. This suggests that B cell development, BCR signaling pathway and C1q expression may be under direct circadian control and dysregulation of which contributes to autoimmunity.

2018-08-24 | GSE87467 | GEO

Gene expression analysis during time dependent Cry toxin exposure in Achaea janata larva.

Project description: Bacillus thuringiensis Cry toxin is used for insect pest management. When susceptible insect larvae ingest the Bt toxin it shows various physiological and cellular changes. We have employed Agilent One Color matrix based microarray to check the mid-gut gene expression upon Cry toxin exposure in Achaea janata (Castor semilooper). Microarray analysis shows significant variation in gene expression upon Cry toxin exposure.

2018-05-26 | GSE114934 | GEO

RNA-seq analysis of cry vvd wc-1 triple knockout(△cry,△vvd,△wc-1) strain in the filamentous fungus neurospora crassa

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Project description:Algae encode up to five different types of cryptochrome photoreceptors. So far, relatively little is known about the biological functions of the so-called DASH (Drosophila, Arabidopsis, Synechocystis and Homo)-type cryptochromes. The green alga Chlamydomonas reinhardtii encodes two of them. CRY-DASH1 also called DCRY1 has its maximal absorption peak in the UV-A range. It is localized in the chloroplast and plays an important role in balancing the photosynthetic machinery. Here, we performed a comparative analysis of chloroplast proteins from wild type and a knockout mutant of CRY-DASH1 named cry-dash1mut, using label-free quantitative proteomics as well as immunoblotting. Our results show upregulation of enzymes involved in specific pathways in the mutant including key enzymes of chlorophyll and carotenoid biosynthesis consistent with increased levels of photosynthetic pigments in cry-dash1mut. There is also an increase in certain redox as well as photosystem I and II proteins, including D1. Strikingly, CRY-DASH1 is coregulated in a D1 deletion mutant, where its amount is increased. In contrast, key proteins of the central carbon metabolism, including glycolysis/gluconeogenesis, dark fermentation and the oxidative pentose pathway are downregulated in cry-dash1mut. Similarly, enzymes of histidine biosynthesis are downregulated in cry-dash1mut leading to a reduction in the amount of free histidine. Yet, transcripts encoding for several of these proteins are at a similar level in the wild type and cry-dash1mut or even opposite. We show that CRY-DASH1 can bind to RNA, taking the the psbA RNA encoding D1 as target. These data suggest that CRY-DASH1 regulates plastidial metabolic pathways at the post-transcriptional level.

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