Project description:This project aims to investigate the effects of 3% oxygen environments on THP-1 cells as compared to 20% oxygen environments. THP-1 cells (pre-seed) were divided into 2 different conditions and cultured in 20% and 3% oxygen environments for 24h. Samples were performed in triplicates.
Project description:Bacteria isolated from potato scab lesions in Finland or northern Sweden were analyzed using microarrays, PCR, and sequencing. Data indicate wide genetic variability in pathogenicity islands among S.turgidiscabies and S.scabies strains.
Project description:Subjects were exposed to three hypoxic environments, a hypobaric altitude chamber (AC), a restricted oxygen breathing device (ROBD), and a restricted oxygen breathing environment (ROBE), and blood was collected during each exposure for total RNA analysis. Microarrays were used to examine the transcriptional response from subjects for each of the three conditions and results were compared. Few differences were noted in gene expresssion when comparing AC, ROBD, and ROBE.
