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Expression data from in vitro polarized-pathogenic Th17 system transfected with YY1 shRNA lentivirus

ABSTRACT: Expression data from in vitro polarized-pathogenic Th17 system transfected with YY1 shRNA lentivirus

PROVIDER: PRJNA767313 | ENA |

REPOSITORIES: ENA

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Expression data from in vitro polarized-pathogenic Th17 system transfected with YY1 shRNA lentivirus

Project description:The proportion of pathogenic Th17 (pTh17) cells were significantly higher in RA than that in control individuals and showed a potential relevance with YY1 expression. Increased YY1 expression was observed in pTh17, and the pTh17 differentiation was hampered by YY1 knockdown. We used microarrays to detail the gene expression changes of pTh17 transfected with YY1 shRNA lentivirus and tried to elucidate the potential mechanisms how YY1 can affect pTh17 cell differentiation in RA.

2021-09-30 | GSE184979 | GEO

Expression data from HCT116 cell transduced with lentivirus encoding PNO1 shRNA (sh-PNO1) or Control shRNA (sh-Ctrl)

Project description:To further explore the underlying mechanism of PNO1 knockdown on suppression of CRC cell growth in vivo and in vitro, Affymetrix human GeneChip primeview arrays were performed to determine the global gene expression in HCT116 cells after transduction lentivirus encoding PNO1 shRNA (n=3) or control shRNA (n=3).

2019-04-23 | GSE113514 | GEO

Expression data from HCT116 cell transduced with lentivirus encoding HAUS6 shRNA (sh-HAUS6) or Control shRNA (sh-Ctrl)

Project description:To further explore the underlying mechanism of HAUS6 knockdown on suppression of CRC cell growth in vivo and in vitro, Affymetrix human GeneChip primeview arrays were performed to determine the global gene expression in HCT116 cells after transduction lentivirus encoding HAUS6 shRNA (n=3) or control shRNA (n=3).

2020-10-01 | GSE140326 | GEO

RNA-seq analysis of human monocytes exposed to lentivirus expressing shRNA against ETV3 or ETV6 during differentiation

Project description:Human monocytes exposed to lentivirus expressing shRNA against ETV3 or ETV6 during differentiation

2022-10-05 | GSE188982 | GEO

Methylated RNA immunoprecipitation sequencing in the Kasumi-1 cell line transfected with WTAP shRNA or scramble control shRNA

Project description:To evaluate the role of WTAP in modulating the m6A abundance, we conducted m6A sequencing in the Kasumi-1 cell line transfected with a lentivirus-mediated short hairpin RNA (shRNA) targeting WTAP gene or a scramble control shRNA.

2022-09-28 | GSE214225 | GEO

Expression data from HCT116 cell transduced with lentivirus encoding PNO1 shRNA (sh-PNO1) or Control shRNA (sh-Ctrl)

Project description:Expression data from HCT116 cell transduced with lentivirus encoding PNO1 shRNA (sh-PNO1) or Control shRNA (sh-Ctrl)

| PRJNA451419 | ENA

Expression data from HCT116 cell transduced with lentivirus encoding HAUS6 shRNA (sh-HAUS6) or Control shRNA (sh-Ctrl)

Project description:Expression data from HCT116 cell transduced with lentivirus encoding HAUS6 shRNA (sh-HAUS6) or Control shRNA (sh-Ctrl)

| PRJNA589313 | ENA

Tumor-specific Th17-polarized cells eradicate large established melanoma

Project description:CD4+ T cells can differentiate into multiple effector subsets but the potential roles of these subsets in anti-tumor immunity have not been fully explored. We sought to study the impact of CD4+ T cell polarization on efficacy of tumor rejection in a model closely mimicking human disease where the target antigens are often non-mutated tissue differentiation self-antigens. We generated a new transgenic mouse model in which CD4+ T cells recognize a novel epitope in tyrosinase-related protein 1 (TRP-1), an antigen that is expressed by normal melanocytes and B16 murine melanoma. We found that cells could be robustly polarized into Th0, Th1 and Th17 subtypes in vitro, as evidenced by cytokine, chemokine, and adhesion molecule profiles as well as by surface markers, suggesting the potential for differential effector function in vivo. Contrary to the current view that Th1 cells play the most important role in tumor rejection, we found that Th17-polarized cells were superior in mediating destruction of advanced B16 melanoma. Unexpectedly, their therapeutic effect was critically dependent on IFN-Î³ production, while depletion of IL-17A and IL-23 had little impact. Taken together, these data indicate that the appropriate in vitro polarization of effector CD4+ T cells is decisive for the successful tumor eradication. This principle should be taken under consideration in the design of future clinical trials involving adoptive transfer-based immunotherapy of human malignancies. Two-condition experiment: RNA from TRP-1 transgenic mouse lymphocytes polarized to be either Th1 or Th17 were compared to cells polarized to be Th0. Keywords: Lymphocyte polarization comparison Comparative analysis of gene expression changes observed when murine lymphocytes are differentially in vitro polarized .

2010-05-25 | E-GEOD-10814 | biostudies-arrayexpress

Assessing the dynamics and macromolecular interactions of the intrinsically disordered protein YY1

Project description:YY1 is a ubiquitously expressed, intrinsically disordered transcription factor involved in neural development. The oligomeric state of YY1 varies depending on the environment. These changes may alter its DNA binding ability and hence its transcriptional activity. In addition to its oligomeric state, the interaction of YY1 with proteins such as FOXP2 can impact its role in transcription. The aim of this work is to study the structure and dynamics of YY1 binding to DNA and to determine the influence of oligomerisation and associations with FOXP2 on its DNA binding mechanism. Size exclusion chromatography, fluorescence anisotropy and electrophoretic mobility shift assays were used to study YY1 oligomerisation and interaction with FOXP2. To better understand potential structural changes to YY1 upon DNA binding, hydrogen deuterium exchange mass spectrometry was used. The results indicate that YY1 consists of specific structured regions, while most of the sequence remains disordered. Furthermore, the oligomeric nature of the protein is dependent on ionic strength. DNA affects oligomerisation and the protein undergoes changes in structure and dynamics upon DNA binding. YY1 and FOXP2 were found to interact with each other both in isolation and in the presence of YY1-specific DNA. The heterogeneous, dynamic multimerisation of YY1 identified in this work is, therefore, likely to be important for its ability to make heterologous associations with other proteins such as FOXP2. The interactions that YY1 forms with itself, FOXP2 and DNA form part of an intricate mechanism of transcriptional regulation by YY1, which is vital for appropriate neural development.

2024-01-26 | PXD038431 | Pride

USP7 shRNA knock-down in T-ALL cells [Jurkat_HSB2_shRNA_RNA-seq]

Project description:RNAseq analysis of USP7 shRNA KD in T-ALL cell lines. USP7 shRNAs lentivirus was generated by co-transfecting 293T cells with shRNA vectors (OriGene or Sigma). T-ALL cells were transduced with USP7 shRNA lentivirus and sorted for GFP positive cells five days after transduction or selected by puromycin.

2021-03-09 | GSE148522 | GEO

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