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Rec8 ChIP-seq for wild type, eco1-aa, wpl1Δ and eco1-aa wpl1Δ strains arrested in meiotic prophase I

ABSTRACT: Rec8 ChIP-seq for wild type, eco1-aa, wpl1Δ and eco1-aa wpl1Δ strains arrested in meiotic prophase I

PROVIDER: PRJNA767385 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16118200_1.fastq.gz	Fastqsanger.gz
	SRR16118200_2.fastq.gz	Fastqsanger.gz
	SRR16118201_1.fastq.gz	Fastqsanger.gz
	SRR16118201_2.fastq.gz	Fastqsanger.gz
	SRR16118202_1.fastq.gz	Fastqsanger.gz

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Chromosome conformation for wild type, eco1-aa, wpl1Δ and eco1-aa wpl1Δ strains arrested in meiotic prophase I

Project description:Chromosome conformation for wild type, eco1-aa, wpl1Δ and eco1-aa wpl1Δ strains arrested in meiotic prophase I

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Chromosome conformation for wild type, eco1-aa, cdc6-mn and eco1-aa cdc6-mn strains arrested in meiotic prophase I

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Chromosome conformation for wild type, eco1-aa, wpl1Δ and eco1-aa wpl1Δ strains arrested in meiotic prophase I

Project description:High-resolution Hi-C in meiotic prophase arrested cells

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Rec8 ChIP-seq for wild type, eco1-aa, wpl1Δ and eco1-aa wpl1Δ strains arrested in meiotic prophase I

Project description:Genome binding/occupancy profiling of the cohesin subunit Rec8 by high throughput sequencing.

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Calibrated Smc3 ChIP-seq for wild type, eco1-aa, wpl1-delta and eco1-aa wpl1-delta strains arrested in meiotic prophase I

Project description:Calibrated Smc3 ChIP-seq for wild type, eco1-aa, wpl1-delta and eco1-aa wpl1-delta strains arrested in meiotic prophase I

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Calibrated Smc3 ChIP-seq for wild type, eco1-aa, cdc6-mn and eco1-aa cdc6-mn strains arrested in meiotic prophase I

Project description:Calibrated Smc3 ChIP-seq for wild type, eco1-aa, cdc6-mn and eco1-aa cdc6-mn strains arrested in meiotic prophase I

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Chromosome conformation for wild type, eco1-aa, cdc6-mn and eco1-aa cdc6-mn strains arrested in meiotic prophase I

Project description:High-resolution Hi-C in meiotic prophase I arrested cells

2022-02-04 | GSE196132 | GEO

Chromosome conformation for wild type and clb5 clb6 strains arrested in meiotic prophase I

Project description:High-resolution Hi-C in meiotic prophase arrested cells

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DNA double-strand breaks regulate the cleavage-independent release of Rec8-cohesin during yeast meiosis

Project description:The mitotic cohesin complex necessary for sister chromatid cohesion and chromatin loop formation shows local and global association to chromosomes in response to DNA double-strand breaks (DSBs). Here, by genome-wide binding analysis of the meiotic cohesin with Rec8 as a kleisin, we found that Rec8 shows dynamic localization from middle to late meiotic prophase I with cleavage-independent global dissociation along chromosomes, driven by meiotic DSB formation. Each Rec8 binding site on the chromosome axis follows distinct dynamics with dissociation and association. Centromeres also showed reduced Rec8 binding in late prophase I relative to mid-prophase I, implying chromosome remodeling of the regions. Rec8 dissociation profile per chromosome is largely correlated with meiotic DSB density. Indeed, the spo11 mutant deficient in meiotic DSB formation did not show the cleavage-independent dissociation of Rec8 in late meiotic prophase I. These suggest the presence of a regulatory pathway for global Rec8-cohesin dynamics in response to meiotic DSBs.

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Rec8 ChIP-Seq experiment to analyse cohesin localisation in the Ctf19c mutants during meiotic prophase.

Project description:Ctf19c mutants were shown to specifically impair cohesin localisation at the pericentromere during mitosis. The goal of this experiment is to confirm whether this mutant has the same effect on cohesin localisation during meiotic prophase. To do so, diploid S. cerevisiae SK1 cells were constructed containing Rec8-3HA in an ndt80-d background. Cells were allowed to sporulate in sporulation media for 5 hours after which formaldehyde was added to the cultures and cells were fixed for 2hr. Samples were then processed according to the standard lab ChIP-Seq protocol (with 3 times 30cycles of 30sec of sonication) Diploid S. cerevisiae SK1 cells were constructed containing Rec8-3HA in an ndt80-d background. Cells were allowed to sporulate in sporulation media for 5 hours after which formaldehyde was added to the cultures and cells were fixed for 2hr. Samples were then processed according to the standard lab ChIP-Seq protocol (with 3 times 30cycles of 30sec of sonication)

2015-12-15 | E-GEOD-70030 | biostudies-arrayexpress

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