Project description:There had a total number of 804 genes with transcript difference, including 482 up-regulated genes and 322 down-regulated genes. Enrichment analysis of those 804 different transcript gene sets was performed to explore the biological effects and the corresponding pathways that are significantly associated on GO and KEGG (Padj <0.05 as the threshold for significant enrichment). In the GO functional enrichment analysis, all differential genes were significantly enriched into 65 GO Terms, among them there had 50 GO terms belonging to BP (Biological Process). Among these BP 50 terms, organonitrogen compound metabolic process (44 genes), organonitrogen compound biosynthetic process (44 genes), and oxidation-reduction process (58 genes) had most different transcript gene sets. While in the MF (Molecular Function), there had 3 GO terms involved transcript changes, which including coenzyme binding (31 genes), structural constituent of ribosome (17 genes) and structural molecule activity (17 genes). In the KEGG pathways enrichment analysis, all differential genes were significantly enriched mainly into 4 pathways, including efq03010 (Ribosome, 30 genes), efq01212 (Fatty acid metabolism, 10 genes), efq00061 (Fatty acid biosynthesis, 10 genes), and efq02010 (ABC transporters, 19 genes), where efq03010 (Ribosome), efq01212 (Fatty acid metabolism), efq00061 (Fatty acid biosynthesis) were up-transcripted pathways, and efq02010 (ABC transporters) was the down-transcripted pathway. Based on transcriptome data analysis in selenium-enriched E. durans A8-1 compared with E. durans A8-1, a total of 19 genes were screened for significant up- transcripted that may be associated with selenium metabolic processes
